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微小核糖核酸-34a在膀胱癌组织中表达的临床意义 被引量:1

Expression and role of miR-34a in bladder cancer
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摘要 目的探讨微小核糖核酸-34a(miR-34a)在膀胱癌组织中表达的临床意义。方法膀胱癌手术切除标本42例,按照UICC2002TNM临床分期系统,非肌层浸润性癌17例,肌层浸润性癌25例;按照WHO1973分级系统,低级别18例,高级别24例。癌旁5cm正常膀胱黏膜标本42例作为对照组。采用荧光实时定量PCR法检测组织中miR-34a的基因表达水平。应用化学方法合成成熟miR-34a瞬时转染膀胱癌T24细胞,流式细胞术检测miR-34a对T24细胞凋亡和生长增殖的影响。结果42例膀胱癌组织标本中miR-34a显著低表达26例(61.9%),不表达11例(26.5%),高表达5例(11.6%),且miR-34a的低表达与肿瘤大小及恶性程度相关,肿瘤越大,恶性程度越高,miR-34a表达量越低。转染miR-34a模拟物后T24细胞生长增殖明显受抑,细胞凋亡率为(9.11±0.41)%,与阴性对照组(3.05±0.29)%和空白对照组(3.73±0.55)%相比差异有统计学意义(P〈0.01)。结论低表达miR-34a可能是膀胱癌组织重要的生物学特征,并参与膀胱癌的生物学进程。 Objective To investigate the expression and biological function of microRNA-34a (miR-34a) in bladder cancer. Methods Forty-two cases of bladder cancer specimen, including 17 non- invasive carcinoma and 25 muscle invasive carcinoma classified by UICC 2002 TNM, or 18 low-grade and 24 high-grade classified by WHO 1973. Meanwhile, mucosa adjacent to the carcinoma was selected as normal control. The gene expression of miR-34a was determined using real-time quantitative polymerase chain reac- tion in 42 cases of bladder carcinoma samples. Mature mimics of miR-34a were chemically synthesized and transiently transfected into T24 bladder cancer cells. The effects of miR-34a on apoptosis and proliferation in T24 cells were evaluated by flow cytometry and MTS respectively. Results 61.9% of carcinoma samples showed low expression of miR-34a, which was correlated with the malignancy and tumor size of bladder car- cinoma. 26.5% (11 cases) was negative and 11.6% (5 cases) showed high expression. Furthermore, up- regulation of miR-34a in T24 cells contributed to cell growth and apoptosis. The apoptosis rate of T24 cells was (9.11 ±0.41 )% , which had significant difference compared with NC mimics and blank control group respectively (P 〈 0. O1 ). Conclusion The relative low expression of miRNA-34a may be involved in the tumorigenesis and development of bladder carcinoma.
出处 《中华泌尿外科杂志》 CAS CSCD 北大核心 2012年第12期939-942,共4页 Chinese Journal of Urology
基金 天津市应用基础与前沿技术研究计划(11JCYBJC14600) 武警后勤学院附属医院种子基金面上项目(FYM201211)
关键词 MIR-34A 膀胱肿瘤 凋亡 MicroRNA-34a Bladder neoplasm Carcinoma Apoptosis
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