摘要
测定了地稔中没食子酸的含量。采用高效液相色谱法，Diamonsil C18色谱柱（250mm×4．6mm，5μm），流动相甲醇-0．1％磷酸溶液（3：97），检测波长273nm，流速为1．0mL·min-1，柱温为室温。结果表明，在5-50μg·mL-1范围内，没食子酸浓度（c）与其峰面积（A）呈良好的线性关系（r=0．9999），平均回收率为99．2％，RSD=0．93％（n=6）。说明该法快速简单，稳定可靠，专属性强。
To determine the content of gallic acid in Melastoma dodecandrum with HPLC. Diamonsil C18 column （250 mm×4.6 mm, 5 μm） was used. The mobile phase was methanol-0.1% phosphoric acid （3 ： 97）, and detected at 273 nm; with flow rate of 1.0 mL.min-1, and the temperature of column was at room temperature. The results showed the linearity of gallic acid was over the range of 5-50 μg.mL-1 with the correlation coefficient 0.999 9. The average recoveries was 99.2% with RSD=0.93% （n=6）. The conclusion indicated the method was convenient, stable, reliable and special for the determination of gallic acid in Melastoma dodecandrum.
出处
《食品工业》
北大核心
2012年第12期176-177,共2页
The Food Industry
基金
江西省高等学校改革研究课题(JXJG-11-14-21)
江西省宜春市社会发展科技计划项目(JXYC2012KSA001)
关键词
没食子酸
高效液相色谱法
含量测定
地稔
gallic acid
HPLC
content determination
Melastoma dodecandrum
