摘要
目的探讨FKS06对糖尿病早期血-视网膜屏障破坏及视网膜神经节细胞的凋亡是否具有保护作用。方法成年雄性Wistar大鼠，链脲佐菌素（Streptozotocin，STZ）腹腔注射诱导糖尿病，取成模后14d大鼠选其中-眼玻璃体腔注射FK5062．5ng作为FK506玻璃体腔注射组；玻璃体注射对照组取成模后14d大鼠任选-眼注射等体积的药物溶剂。手术后48h取材应用免疫荧光技术检测视网膜铺片Occludin的表达改变，TUNEL法检测视网膜神经节细胞凋亡情况。结果糖尿病大鼠FK506注射组视网膜毛细血管及动脉中Occludin表达呈梭形网状排列，排列紊乱、中断，在胞浆内即网眼中可伴点状染色，玻璃体注射对照组视网膜毛细血管及动脉中荧光强度较弱，网眼的排列更为紊乱，中断现象严重。玻璃体注射对照组视网膜节细胞层见少量的凋亡细胞（9．2±3．4）个，切片，FK506注射眼未发现凋亡细胞，两组比较差异显著有统计学意义。结论FK506可增加0ccludin的表达及维持其正常分布，并可抑制视网膜神经节细胞凋亡，我们认为其对糖尿病引起的视网膜损害有保护作用。
Objective To explore the protective effect of FK506 on the blood-retinal barrier dam- age and cell apoptosis in early stage diabetic retinopathy. Methods Streptozotocin （STZ） was intra- peritoneally injected into adult Wistar rats to induce diabetes. At 14 days after induced diabetes, one eye of each diabetic rat was intravitreally injected FK506 2.5ng, the control group was intravitreally injected with 0.08%~ alcohol served as control intravitreal injection group. Forty-eight hours after in- jection, the retina was flatmounted, and the Occludin was measured by immunofluorescence to evalu- ate the change of the blood-retinal barrier. The frozen sections were prepared. And three sections with 300 m interspace were collected on each slide. Each slide was measured by TUNEL to test the apoptosis of retinal cells and the number of positive cells was counted. The data was statistical analyzed by SPSS software. Results Diabetic rats with FK506 intravitreal injection showed that the distribution of Occludin in the capillary and artery were interrupted and in disorder. But there were more interruption and disorder in the control eye. There were a few apoptosis cells （9.2±3.4/slide） in the retinal layer of the control group, and there was no apoptosis cell in the group with FK506. There was significant difference between two groups. Conclusions The expression of Occludin could be regulated by FK506. Furthermore the cell apoptosis caused by diabetic can be protected by it.
出处
《中国实用眼科杂志》
CSCD
北大核心
2012年第12期1508-1511,1512,共5页
Chinese Journal of Practical Ophthalmology
