摘要
目的研究血吸虫重组 BCG- Sj2 6 GST疫苗对小鼠血清 NO浓度的影响。方法第 1次实验用 10 6 和 10 8CFU疫苗分别皮下注射免疫 BAL B/C鼠 ,免疫后 8周用日本血吸虫尾蚴攻击 ,感染后 6周剖杀小鼠 ,同时设有 PBS对照组 ;第 2次实验用 10 6 CFU疫苗皮下和静脉分别注射免疫鼠 ,于免疫后 0、4、8、10、14和 16周各剖杀 4只 ,分离血清 ,用 NO试剂盒检测小鼠血清 NO浓度。结果发现疫苗免疫 ,尾蚴攻击后血清 NO浓度降低 ;疫苗皮下和静脉注射免疫后 4～ 16周血清 NO浓度升高 ,分别于免疫后 10周或 16周达最高水平。结论血吸虫重组 BCG- Sj2 6 GST疫苗能降低感染鼠血清 NO浓度 ,提高宿主抗血吸虫感染的保护力。
Objective To study effects of recombinant BCG Sj26GST vaccine of schistosome on NO concentration of serum in mice.MethodsIn the first experiment,BALB/C mice were immunized subcutaneously by 10 6 and 10 8 CFU vaccine respectively ,challenged with Sj cercariae on 8 weeks of immunization and killed on 6 weeks of infection to separate sera,PBS served as control;In the second experiment,after immunized subcutaneously and intravenously by 10 6 CFU vaccine respectively,4 mice were killed to separate sera on 0,4,8,10,14 and 16 weeks of immunization.NO concentration of serum were detected by NO kit.ResultsIt has been found that NO concentration decreased by immunization with rBCG Sj26GST vaccine against challenge with Sj cercariae;dynamic observation showed NO concentration increased on 4～16 weeks,reached the highest level on 10 weeks and 16 weeks respectively in the subcutaneous and intravenous group.ConclusionrBCG Sj26GST vaccine of schistosome may decrease NO concentration in sera of infected mice,and increase host’s protection against schistosomiasis. [
出处
《免疫学杂志》
CAS
CSCD
北大核心
2000年第4期272-274,共3页
Immunological Journal
