摘要
目的 研究病理性瘢痕成纤维细胞Fas受体及凋亡相关蛋白bcl-2的表达，探讨增生性瘢痕成纤维细胞对Fas介导凋亡的抑制作用。方法 取手术切除的增生性瘢痕和瘢痕疙瘩组织各6例，通过细胞培养6～10代后，应用流式细胞仪、粘附式细胞仪分别检测增生性瘢痕及瘢痕疙瘩成纤维细胞Fas及Bcl-2蛋白的表达。结果 增生性瘢痕成纤维细胞Fas受体呈低表达，而瘢痕疙瘩成纤维细胞Fas受体强表达。凋亡抑制蛋白Bcl-2的表达在增生性瘢痕和瘢痕疙瘩成纤维细胞内均呈低表达，两者无显著差异。结论 正常情况下，Fas Mcab能诱导Fas受体表达阳性的细胞凋亡，瘢痕疙瘩成纤维细胞却表现为Fas受体高表达而不凋亡，Bcl-2低表达。实验结果表明，瘢痕疙瘩成纤维细胞不凋亡现象并非Fas受体缺陷或外源性抑制所致，提示瘢痕疙瘩成纤维细胞膜表面高表达的Fas受体可能处于无功能状态。
Objective To investigate the expression of apoptosis-related protein Fas and Bcl-2 and to explore the corresponding mechanism. Methods Six samples of hypertrophic scars and 6 of keloids were collected cultured, and only 6～10 passages fibroblasts were selected for experiment. With the help of the flow cytometer and adherent cell analysis and sorting interactive laser coytometer (ACAS 570), the expression of apoptosis-related protein -Fas and Bcl-2 were detected. Results The expression of Fas antigen in the fibroblasts derived from the keloids were significant elevated in comparison with that in the fibroblasts from hypertrophic scars. The levels of the Bcl-2 protein, loe in both groups, did not differ significantly between the 2 groups, respectively. Conclusions In contrast to that hypertrophic scars from fibroblasts derived from keloids showed high expressed Fas antigen and resistance to apoptosis induced by FasMcab. Considering the low expression of Bcl-2 protein, we assume that Fas receptor on firoblasts derived from keloids may be insert for functioning.
出处
《第一军医大学学报》
CSCD
2000年第3期231-232,235,共3页
Journal of First Military Medical University
基金
国家自然科学基金(39870807)
