摘要
目的建立工作场所空气中毒死蜱的高效液相色谱测定方法。方法采用硅胶管吸附空气中的毒死蜱，用乙腈解吸，高效液相色谱一紫外检测器测定。结果该方法在0—10．0μg／ml范围内呈线性关系，回归方程为y=5206．1x-104．7，相关系数r=0．9999。检出限为0．006μg／ml，最低检出浓度为0．001mg/m3（以采集4．5L空气样品计）；平均加标回收率为98．3％～102．5％；批内精密度为1．96％。4．39％，批间精密度为2．76％～5．87％；平均解吸效率为99．0％-103．3％，采样效率为94．0％，样品在室温下至少可保存15d。结论该方法各项指标均符合GBZ／T210．4．2008《工作场所空气中化学物质测定方法》的要求，适用于工作场所空气中毒死蜱的检测。
Objective To establish the method of detecting the concentrations of chlorpyrifos in air of workplace with high performance liquid chromatographic （HPLC）. Methods According to standards of methods for determining the chemical substances in workplace air, chlorpyrifos in the air was collected by silicone tube,then dissolved by acetonitrile and determined by high performance liquid chromatography with UV-detector. Results There was a linear relationship within the range of 0 -10.0 μg/ml, and regression equation was y=5206.1x-104.7, correlation coefficient was 0.9999, the detection limit was 0.006 μg/ml. The lowest detected concentration was 0.001 mg/m3 （sampling volume 4.5 L）. The average recoveries was 98.3%- 102.5% . The within-run precision was 1.96% -4.39%, the between-run precision was 2.76% -5.87%. The desorption efficiencies were 99.0%-103.3% and the sampling efficiencies were 94%. The samples in silicone tube could be stored for 15 days at room temperature. Conclusion The present method could meet with the requirements of Guide for establishing occupational health standards-Part 4 Determination methods of air chemicals in workplace and be feasible for determination of chlorpyrifos in workplace air.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2012年第12期953-955,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
毒死蜱
工作场所
色谱法
高效液相
JWorkplace air
Chlorpyrifos
High performance liquid chromatography
