摘要
目的:观察具有化瘀散结功效的金喉片对喉癌裸鼠移植瘤及癌瘤中致癌基因Survivin和抑癌基因PTEN的影响。方法:将培养好的喉癌细胞接种到随机分组的32只雌雄各半裸鼠背部皮下,按折算后的金喉片常规有效剂量的1倍、3倍和6倍分别对裸鼠进行灌胃,并设立生理盐水对照组(空白对照组),观察裸鼠背部瘤体变化。在第28天取瘤,并进行瘤体重量和体积检测,分析瘤体切片中致癌基因Survivin和抑癌基因PTEN的表达差异。结果:高剂量组裸鼠背部皮下喉癌移植瘤体积最小,重量最轻,瘤体的平均密度为1.202g/cm3;Sur-vivin和PTEN在瘤体中的阳性率均体现出高剂量组<中剂量组<低剂量组<空白对照组的规律特点。结论:6倍等效剂量的金喉片能够有效抑制喉癌裸鼠移植瘤的生长,并且降低致癌基因Survivin在癌瘤中的表达,抑癌基因PTEN的阳性表达始终低于Survivin,体现出一种从属表达的特征。
Objective： To explore the positive expressing rates of oncogene Survivin and tumor-suppressor gene PTEN in transplanted laryngocarcinoma of nude mice treated by Gold Throat Tablets（GTT） which can improve circulation and remove haemostasis in TCM theory. Method：The 32 nude mice seeded with cultured laryngeal carci- noma cells subcutaneously at the back were randomly divided into high, middle, low（according to 6 ： 3 ： 1 propor- tion of GTT dosing given by intragastric administration） dose groups and blank control groups. The changes on weight and size of tumors originated from these cells were observed for 28 days, and the density of tumors and ex- pression of Survivin and PTEN were examined with tumor sections by immunohistochemical assay after separating tumors from back of nude mice. Result： The weight and size of subcutaneous laryngocareinoma on backs of high dose group nude mice were both the smallest among all the experimental groups with the average density of tumors as 1. 202 g/cm3. The positive expressing rates of Survivin and PTEN both revealed the following tendency that high close group〈〈middle close group〈~low dose group（blank control group. Conclusion：Six times of regular doses of GTT can prevent overgrowth of laryngocarcinoma transplanted on nude mice and decrease the excessive expres- sion of oneogene Survivin in the tumor. PTEN, expressing lower than Survivin in all groups, shows a subordina- tive relationship with it, maybe due to a competition mechanism.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第24期1134-1137,1143,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
广东省科技厅项目(No:2008B030301213)
