摘要
采用薄层色谱法对四数九里香药材中补骨脂素定性鉴别,高效液相色谱法测定补骨脂素含量,建立四数九里香中补骨脂素的定性定量分析方法。色谱柱为大连依力特C18柱(4.6mm×250mm,5μm),流动相为甲醇-水(52∶48),流速1.0ml/min;检测波长246nm。结果表明,TLC斑点清晰,补骨脂素在0.06～0.36μg范围内呈良好线性关系(r=0.9992),平均回收率为101.6%,RSD为1.84%(n=6)。该方法准确、可靠,可以作为四数九里香药材的质量标准制定分析方法。
The qualitative and quantitative methods were established to estimate psoralen in Murraya tetraera Huang. Murraya tetraera Huang was identified by using TLC and the con- tent of psoralen in the material was determined by HPLC. SinoChrom ODS-BP column （4.6 mm×250 mm,5 μm） was adopted with methol-water （52 ： 48） at the flow of 1.0 ml/min,and the detection wavelength was 246 nm. The results showed that the TLC spots were clear,and the calibration curve of psoralen was linear within the range of 0.06~0. 36μg, r =0. 9992; the average recovery was 101.6% and RSD was 1.84% （n =6）. The method is accuracy and relia- ble,and can be adopted as a standard for the quality control of Murraya tetraera Huang.
出处
《广西科学》
CAS
2012年第4期352-354,共3页
Guangxi Sciences
基金
广西中药质量标准研究重点实验室系统性研究课题(桂中重系201209)资助
