摘要
壳聚糖与芳香醛反应得到壳聚糖席夫碱,将席夫碱与氯乙酸反应后的产物去除氨基保护得到o-羧甲基壳聚糖。产物经FT-IR、1H-NMR结构表征,证实所得产物为目标产物;产物的TG和DTG分析结果与壳聚糖相比差异明显,热稳定性相差较大;抑菌性研究表明,新法制备的壳聚糖衍生物对金黄色葡萄球菌和黑曲霉菌抑制能力明显大于壳聚糖,对两种菌种的最小抑菌浓度均达0.5%。由于o-羧甲基壳聚糖良好的溶解性及抗菌能力,预计在食品、医药等领域会发挥较大优势。
The Schiff base of chitosan was obtained by the reaction of chitosan with aromatic aldehydes, then the obtained Schiff base of chitosan reacts with chloroacetic acid, and finally, the amino protection group of the product was removed to get the chitosan derivative. The obtained chitosan derivative was characterized by FT-IR and ^1H-NMR, and the results show that the obtained chitosan derivative is the target product, the o-carboxymethyl-chitosan. The analysis results of TG and DTG show that the thermal stability of the obtained chitosan derivative is obviously different from that of chitosan. The antimicrobial properties of the chitosan and obtained chitosan derivative were analyzed by their antimicropial activities against Staphylococcus aureus and Aspergillus niger. The results indicate that both the antimicrobial activities of the chitosan derivative and chitosan increase with the increase of their concentrations, and the antimicrobial activities of the chitosan derivative are stronger than that of chitosan. The minimum inhibitory concentration （MIC） of the chitosan derivative against Staphylococcus aureus and Aspergillus niger was found to be 0.5%. Since the solubility of the chitosan derivative is good in organic solvent, it could be expected to have a big advantage of to be used in the fields of food and medicine.
出处
《高校化学工程学报》
EI
CAS
CSCD
北大核心
2012年第6期1048-1053,共6页
Journal of Chemical Engineering of Chinese Universities
基金
科技部国际科技合作重点资助项目(2004DFA03600)
留学回国人员科研启动基金项目
关键词
席夫碱
结构表征
热稳定性
抗菌性
Schiffbase
structure characterization
thermostability
antimicrobial activities
