摘要
首次采用紫外诱变和喹禾灵筛选的方法,选育出2株二十二碳六烯酸(Docosahexaenoic acid,DHA)含量高的裂殖壶菌(Schizochytrium)突变菌株OUC002和OUC007,其中OUC002生物量(6.82g·d-1·L-1)和DHA含量(15.31%),分别比对照菌株OUC168提高8.08%和13.74%;OUC007生物量(7.04g·d-1·L-1)和DHA含量(17.33%),比对照菌株分别提高11.57%和28.75%。通过GC-MS(气质联用)对OUC007脂肪酸组成进行分析,DHA含量达到脂肪酸的37.28%。本研究为裂殖壶菌的发酵生产提供了性状优良的菌种,并为裂殖壶菌菌种选育提供了新方法。
Schizochytriurn limacinum was mutated with ultraviolet and two elite strains, OUC002 and OUC007, were screened out with quizalofop-p-ethyl. Compared with original strain, the biomass and DHA content of OUC002 were increased by 8. 08% and 13. 74%, respectively, and the biomass and DHA content of OUC007 were increased by 11. 57% and 28. 75%, respectively. As determined with GC-MS, DHA content of OUC007 reached 37. 28% of total fatty acids. These mutants promise to be used in S. limacinum fermentation. The method used in this study was effective for breeding new strains of S. limacinum.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第12期54-58,共5页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目(2008AA09Z410)
国家科技支撑计划项目(2006BAD09A12)资助
关键词
裂殖壶菌
喹禾灵
紫外线
脂肪酸组成
DHA
Schizochytrium limacinum
quizalofop-p-ethyl
ultraviolet
fatty acid composition
DHA
