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Erlotinib对鼻咽癌细胞株放射敏感性的作用 被引量：2

Role of Erlotinib in radiosensitivity of nasopharyngeal carcinoma cell lines
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摘要 目的:研究Erlotinib对人鼻咽癌细胞株CNE1及CNE2放射敏感性的影响。方法:人鼻咽癌细胞株CNE1、CNE2经Erlotinib、深部X线照射或两者联合处理,采用细胞克隆形成法检测Erlotinib对鼻咽癌细胞株放射敏感性的影响;流式细胞仪检测细胞凋亡及细胞周期的情况。结果:Erlotinib增强了鼻咽癌细胞株CNE1、CNE2的放射敏感性,放射增敏比分别为1.076、1.109;Erlotinib联合电离辐射可导致鼻咽癌细胞株CNE1、CNE2发生G2/M期阻滞;并促进鼻咽癌细胞株CNE2放射诱导的细胞凋亡。结论:Erlotinib联合电离辐射可致鼻咽癌细胞周期G2/M期阻滞,增强了鼻咽癌细胞株的放射敏感性。 Aim：To explore the role of Erlotinib in radiosensitivity of human nasopharyngeal carcinoma CNE1 and CNE2 cell lines. Methods： Human nasopharyngeal carcinoma cells dealed with Erlotinib and/or Radiation.The effect of Erlotinib on radiosensitivity was detected by using clonogenic survival assay.Cell apototic rate and cell cycle were evaluated by flow cytometery. Results： Erlotinib enhanced radiation sensitivity of CNE1 and CNE2 cells,the SER was 1.076 and 1.109.Erlotinib combine with radiation induced G2 phase arrest of two cells,only induced the apoptosis of CNE2 cell. Conclusion：Erlotinib combine with radiation provoked G2 phase arrest enhanced radiation sensitivity.
作者 张秀萍 张欢欢 袁太泽 梁颖 黄赖机 李健 叶家才 郑荣辉
机构地区 广州医学院附属肿瘤医院放疗科
出处 《暨南大学学报（自然科学与医学版）》 CAS CSCD 北大核心 2012年第6期579-582,590,共5页 Journal of Jinan University(Natural Science & Medicine Edition)
基金 广州市科技局项目(2009Z1-E281)
关键词 鼻咽癌 电离辐射 表皮生长因子受体 厄洛替尼 nasopharyngeal carcinoma radiation EGFR Erlotinib
分类号 R319 [医药卫生—基础医学]
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二级参考文献32

  • 1徐美林,刘岩,钟镐镐,吴秉铨.非小细胞肺癌表皮生长因子受体突变的检测及临床病理意义[J].中华病理学杂志,2007,36(7):453-456. 被引量：9
  • 2Chan A T, Teo P M, Ngan R K, et al. Concurrent chemotherapy-radiotherapy compared with radiotherapy alone in locoregionally advanced nasopharyngeal carcinoma: progression-free survival analysis of a phase Ⅱ randomized trial [J]. J Clin Oncol, 2002,20(8):2038-2044.
  • 3AI-Sarraf M, LeBlanc M, Giri P G, et al. Chemoradiotherapy versus radiotherapy in patients with advanced nasopharyngeal cancer: phase Ⅲ randomized intergroup study 0099 [J]. J Clin Oncol, 1998,16(4) : 1310-1317.
  • 4Teo P, Yu P, Lee W Y, et al. Significant prognosticators after primary radiotherapy in 903 nondisseminated nasopharyngeal carcinoma evaluated by computer tomography [J]. Int J Radiat Oncol Biol Phys, 1996,36(2):291-304.
  • 5Schlessinger J. Cell signaling by receptor tyrosine kinases [J]. Cell, 2000,103(2) :211-225.
  • 6Raben D, Helfrich B A, Chan D, et al. ZD1839, a selective epidermal growth factor receptor tyrosine kinase inhibitor, alone and in combination with radiation and chemotherapy as a new therapeutic strategy in non-small cell lung cancer [J]. Semin Oncol, 2002,29(1 Suppl 4) :37-46.
  • 7Cohen S. The epidermal growth factor (EGF) [J]. Cancer, 1983,51 (10) : 1787-1791.
  • 8Derynck R. Transforming growth factor alpha [J]. Cell, 1988, 54 (5) : 593-595.
  • 9Pivot X, Magne N, Guardiola E, et al. Prognostic impact of the epidermal growth factor receptor levels for patients with larynx and hypopharynx cancer [J]. Oral Oncol, 2005,41 (3) :320-327.
  • 10Li S, Kim J S, Kim J M, et al. Epidermal growth factor receptor as a prognostic factor in locally advanced rectalcancer patients treated with preoperative chemoradiation[J].Int J Radiat Oncol Biol Phys, 2006,65(3):705-712.

共引文献18

同被引文献40

  • 1LEE A W, LAU W H, TUNG S Y, et al. Preliminary results of a randomized study on therapeutic gain by concurrent chemotherapy for regionally - advanced nasopharyngeal carcinoma: NPC - 9901 Trial by the Hong Kong Nasopharyngeal Cancer Study Group [J]. J Clin Oncol, 2005, 23 ( 28) : 6966 - 6975.
  • 2GIACCONE G, GALLEGOS R M, LE C T, et al. Erlotinib for frontline treatment of advanced non - small cell lung cancer: a phase II study [J]. CJin Cancer Res, 2006, 12 (20 PI 1) : 6049 - 6055.
  • 3WANG M, MORSBACH F, SANDER D, et al. EGF receptor inhibition radiosensitizes NSCLC cells by inducing senescence in cells sustaining DNA double - strand breaks [J]. Cancer Res, 2011, 71 (19): 6261 -6269.
  • 4BOZEC A, SUDAKA A, FISCHEL J L, et al. Combined effects of bevacizumab with erlotinib and irradiation: a preclinical study on a head and neck cancer orthotopic model [J]. Br J Cancer, 2008, 99(1) : 93 -99.
  • 5BECK T, WEIKEL W, BRUMM C, et a!. Immunohistochemical detection of hormone receptors in breast carcinomas (ER - ICA, PgR - ICA): prognostic usefulness and comparison with the biochemical radioactive - ligand - binding assay (DCC) [J]. Gynecol Oncol, 1994, 53 (2) : 220 - 227 .
  • 6CHUA D, SHAM J S, AU G K. A concurrent chemoirradiation with cisplatin followed by adjuvant chemotherapy with ifosfamide, 5 - fluorouracil, and leucovorin for stage N nasopharyngeal carcinoma[J]. Head Neck, 2004, 26(2): 118 -126.
  • 7HITT R, CIRUELOS E, AMADOR M L, et a!. Prognostic value of the epidermal growth factor receptor (EGRF) and p53 in advanced head and neck squamous cell carcinoma patients treated with induction chemotherapy [J]. Eur J Cancer, 2005, 41 (3 ) : 453 -460.
  • 8MA B B, POON T C, TO K F, et a!. Prognostic significance of tumor angiogenesis, Ki 67, p53 oncoprotein, epidermal growth factor receptor and HER2 receptor protein expression in undifferentiated nasopharyngeal carcinoma-a prospective study [J]. Head Neck, 2003, 25(10): 864 -872.
  • 9CHINNAIYAN P, HUANG S, VALLABHANENI G, et al. Mechanisms of enhanced radiation response following epidermal growth factor receptor signaling inhibition by erlotinib (Tarceva) [J] . Cancer Res, 2005, 65 (8) : 3328 - 3335.
  • 10MORGANMA, PARSELSLA, KOLLARLE, eta!' Thecombination of epidermal growth factor receptor inhibitors with gemcitabine and radiation in pancreatic cancer [J]. Clin Cancer Res, 2008, 14(16): 5142 -5149.

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二级引证文献8

暨南大学学报（自然科学与医学版）
2012年 第6期

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