摘要
建立了高效液相色谱紫外检测器测定豆制品中碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O的方法。样品用乙腈-水(7+3)溶液提取,经高效液相色谱仪紫外检测器测定。结果表明:碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O在0.1～20μg/mL范围内呈良好线性关系,相关系数均为0.9999,3个水平(5.0、10.0、15.0mg/kg)加标回收率为88.8%～93.0%,相对标准偏差为0.67%～1.66%(n=6),检出限分别为碱性橙Ⅱ:0.02 mg/kg、酸性橙Ⅱ:0.05 mg/kg和碱性嫩黄O:0.05 mg/kg。此方法具有简单、快速、准确等优点,适用于豆制品中碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O的同时测定。
High performance liquid chromatography（HPLC） with UV detector method was developed tbr the analysis of Basic Orange Ⅱ, Acid Orange Ⅱ and Auramine O in bean products. Samples was extracted by acetonitrile and water（7＋3）, and determined by HPLC with UV detector. The calibration curve of the method was linear over the range of 0.1 to 20.0 μg/mL with a correlation coefficient of 0.9999. The recoveries for Basic Orange Ⅱ, Acid Orange Ⅱ and Auramine O standard spiked in the range of 5.0 to 15.0 mg/kg were between 88.8 % to 93.0 % with a relative standard deviation （RSD） being between 0.67 % to 1.66 %（n=6）. The detection limit was Basic Orange 11 ： 0.02 mg/kg, Acid Orange Ⅱ： 0.05 mg/kg, Auramine O： 0.05 mg/kg. Owing to advantages of good linearity, high precision and rapidity, this method is suitable FOr the determination of Basic Orange Ⅱ, Acid Orange Ⅱ and Auramine O in bean products.
出处
《广东化工》
CAS
2012年第18期127-128,145,共3页
Guangdong Chemical Industry
