摘要
目的建立黄芩中的六六六(BHC)、滴滴涕(DDT)和五氯硝基苯(PNCB)3种农药残留量的毛细管气相色谱测定方法。方法样品采用丙酮-二氯甲烷为提取溶剂,以超声法提取,采用磺化法净化。检测使用弹性毛细管柱为分离柱,电子捕获检测器(ECD)检测。结果 3种成分在25min内得到良好的分离;两个色谱峰之间的分离度最小为1.57,峰与峰之间达到良好分离;该法中9个农药残留物组分的线性关系良好,其平均加样回收率为72.20%～116.18%,RSD值为0.55%～7.81%。结论该法准确,灵敏度高,易操作,重复性良好。
Objective To establish a capillary gas chromatographic（GC） method to determine residues of benzene hexachloride（BHC）,dichloro-diphenyl-trichloroethane（DDT）,and pentachloronitrobenzene（PCNB） in Scutellariae Radix.Methods The pesticide in samples was extracted online with acetone-dichloromethane as extraction solvent,using ultrasonic extraction and sulfonating purification,petroleum ether as final solvent.Capillary column was used as detached dowel,and the determination was carried out by electron capture detector.Results The three pesticides were separated fully in 25 min.The number of theoretical plates complied with requirements in Chinese Pharmacopoeia;the minimum resolution is 1.571,any peaks were separated completely.There was a good linear relationship among the nine pesticide residues.The average recovery rate was 72.20%—116.18%,and RSD values were 0.55%—7.87%.Conclusion The method is accurate,highly sensitive,easy in operation,and has good reproducibility.
出处
《药物评价研究》
CAS
2012年第6期443-446,共4页
Drug Evaluation Research
关键词
毛细管气相色谱法
黄芩
农药
残留量
capillary gas chromatograph
Scutellariae Radix
pesticide
residue
