摘要
本研究旨在探讨髓过氧化物酶(MPO)和醌氧化还原酶1(NQO1)基因多态性与中国甘肃人群急性白血病易感性的关系。用1∶1配对病例-对照研究和连接酶检测反应(LDR)分型方法分析急性白血病病例组(150例)和对照组(150例无癌住院患者)MPO和NQO1的基因多态性,比较不同基因型与急性白血病易感性的关系。结果表明,病例组MPO-463A等位基因分布频率低于对照组,MPO(G-463A)各基因型在病例组与对照组中的分布差异显著(χ2=11.828,P<0.05,OR=0.368,95%CI=0.205-0.610)。病例组NQO1-609T等位基因分布频率高于对照组,NQO1(C-609T)各基因型在病例组与对照组中的分布差异显著(χ2=17.931,P<0.05,OR=1.428,95%CI=1.237-3.339)。基因多态联合作用分析显示,MPO野生型兼具NQO1野生型者发生急性髓系白血病的风险降低至33.6%。结论:MPO、NQO1与急性白血病易感性相关,携带MPO(G-463A)突变基因型(GA/AA)可降低白血病的发病风险,携带NQO1(C-609T)突变基因型(TC/TT)可增加白血病的发病风险,MPO野生型与NQO1野生型者联合作用可进一步降低急性髓系白血病的发病风险。
The aim of this study was to investigate the relationship of the gene polymorphisms of myeloperoxidase （MPO） and NAD （P）H：quinone oxidoreductase 1 （NQO1） with the susceptibility to acute leukemia（AL） in Chinese Gansu population. A 1：1 paired case-control study of 150 patients with acute leukemia and 150 cancer-free inpatients as a control was conducted to detect the polymorphisms of MPO and NQ01 by LDR techniques. The results showed that the MPO-463A genotype frequency in patient group was lower than that in control group, and there was significant difference of MPO （G-463A） genotype between patient group and control group （ X^2 = 11. 828, P 〈 0.05, OR = 0.368, 95 % CI = 0.205 -0.610）. The NQ01-609T genotype frequency in patient group was higher than that in control group, and there was significant difference of NQO1 （C-609T） genetype between patient group and control group （ X^2 = 17.931, P 〈 0.05, OR = 1. 428, 95% CI = 1. 237 - 3. 339）. The combined gene analysis showed that the AML risk in patients carry- ing the wild genotypes of MPO and NQ01 was dropped to 33.6%. It is concluded that the MPO and NQ01 gene poly- morphisms are associated with susceptibility to AL. The AL risk may decrease in patients carrying MPO （G-463A） mu- tant gene（ GA/AA）, while the AL risk may increase in patients carrying NQO1 （C-609T） mutant gene（TC/TT）. The combined effect of MPO and NQ01 wild genotypes may further decrease AL risk.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2012年第6期1336-1340,共5页
Journal of Experimental Hematology
基金
甘肃省科技支撑项目(0708NKCA113)
兰州大学医学科研基金资助项目(LZUYX2008)
