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中国哈尔滨地区献血人群RhDel表型的分子机理研究 被引量：8

Molecular Mechanism of RhDel Phenotype in Blood Donation Population of Chinese Harbin Area
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摘要 本研究探讨中国哈尔滨地区献血人群RhDel表型表达的分子机制。应用血清学及分子生物学方法,对374例经间接抗人球蛋白试验确认为RhD阴性的献血者进行红细胞RHD血型基因分型和RH基因变异体检测,并对无法确定RHD基因型别的特殊样本进行测序分析。结果表明,374例阴性献血者样本中共检出RHD Del型62例,占16.6%。其中RHD 1227A型61例,发现中国人中罕见的RHD Del(cde)1等位基因1例。经测序分析,检出存在RHD711DelC等位基因者11例。另外,在3份样本中发现了新等位基因突变点。结论:RHD1227A等位基因是中国哈尔滨地区Del表型人群所普遍携带的等位基因,是Del表型个体的重要遗传标记。 This study was aimed to explore the molecular mechanism of RhDel phenotype expression in blood donors of Chinese Harbin area. Three hundred and seventy-four RhD negative donors confirmed by indirect antiglobulin test were detected by using serological and molecular methods for red blood cell RhD blood group genotyping and RH gene variant detection. And the other special samples of undetermined RhD genetype were sequenced. The results showed that 62 cases of Del type were detected in 374 negative blood donors＇ samples, accounting for 16.6%, among them there were 61 cases of RHD1227A, and 1 case of rare RHD Del （cde）l allele was found. Sequencing analysis indicated that RHD711DelC allele existed in 11 cases. In addition, the new mutant alleles were found in 3 samples. It is concluded that RHD1227A allele is generally carried allele in Del phenotype population of Chinese Harbin area and is an important genetic marker in Del phenotype individual.
作者 李继红 张春燕 孙建华 刘杰
机构地区 哈尔滨红十字中心血站
出处 《中国实验血液学杂志》 CAS CSCD 北大核心 2012年第6期1478-1481,共4页 Journal of Experimental Hematology
基金 哈尔滨市科技局青年科技创新人才项目(编号:RC2010QN004069)
关键词 RhDel表型 RHD1227A等位基因 中国哈尔滨地区 RhDel phenotype RhD1227A allele Chinese Harbin area
分类号 R457.11 [医药卫生—治疗学]
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参考文献17

  • 1Okuda H, Suganuma H, Tsudo N, et al. Sequence analysis of the spacer region between the RHD and RHCE genes. Bioehem Biophys Res Comman, 1999 ;263:378 - 380.
  • 2Legler TJ , Maas JH, KEh lerM, et al. RHD sequencing: a new tool for decision making on transfusion therapy and provision of Rhprophy laxis. Transfusion Med, 2001 ;11 (5) : 383 -388.
  • 3Okubo Y, Yamaguchi H, Tomita T, et al. A D variant, Del. Transfusion, 1984 ;24 ( 6 ) : 542.
  • 4Kim JY, Kim SY, Kim CA, et al. Molecular characterization of D Korean persons:development of a diagnostic strategy. Transfusion, 2005 ;45(3) :345 -352.
  • 5Okuda H, Kawano M,Iwamoto S, et al. The RHD gene is highly detectable in RhD-negative Japanese donors. J Clin Invest, 1997; 100(2) :373 -379.
  • 6Shao CP,Maas JH, Su YQ, et al. Molecular background of Rh D- Positive, D-negative, Del and weak D phenotypes in Chinese. Vox Sang,2002 ;83 (2) :156 - 161.
  • 7Wagner FF,Frohmajer A, Flegel WA. RhD positive haplotypes in D-negative Eurooeans. BMC Genet .2001:2( 1 ) : 10 - 15.
  • 8Chen JC, Lin TM, Chen YL, et al. RHD 1227A is an importantgenetic marker for RhDel individuals. Am J Clin Pathol, 2004; 122(2) :193 -198.
  • 9Chang JG, Wang JC, Yang TY, et al. Human RhDel is caused by a Deletion of 1,013 bp between introns 8 and 9 including exon 9 of RHD gene. Blood, 1998; 92 (7) : 2602 -2604.
  • 10Richard M, Perreault J, Yanez J, et al. A new DEL variant caused by exon 8 Deletion. Transfusion, 2007, 47(5) : 852-857.

二级参考文献23

  • 1邵超鹏,李桢,熊文,周一炎,李雪梅.cis基因交换形成RHD-CE(2～9)-D等位基因[J].遗传,2005,27(4):561-565. 被引量：11
  • 2中华人民共和国卫生部.中国输血技术操作规程（血站部分）[M].天津科学技术出版社,1997.24-26.
  • 3Mak K H, Yan K F, Cheng S S et al . Rh phenotypes of Chinese blood donors in Hong Kong, with special reference to weak D antigens[J].Transfusion, 1993;3:348.
  • 4Fukumori Y, Hori Y, Ohnoki S et al. Further analysis of D^el (D-elute) using polymerase chain reaction (PCR) with RHD gene-specific primers[J]. Transfus Med, 1997;7:227.
  • 5Sun C F, Chou C S, Lai N C et al. RHD gene polymorphisms among RhD-negative Chinese in Taiwan[J]. Vox Sang, 1998;75:52.
  • 6Legler T J, Maas J H, KohlerM et al . RHD sequeacing: a new tool for decision making on transfusion therapy and provision of Rh prophylaxis[J]. Transfus Med, 2001;11.383.
  • 7Altschul S F, Gish W, Miller Wet 02 . Basic local alignment search tool[J].J Mol Biol, 1990;215:403.
  • 8Legler T J, Maas J H, Blaschke V et al . RHD genotyping in weak D phenotypes by multiple polymerase chain reactions [ J ]. Transfusion,1998;38:434.
  • 9Shao C P, Maas J H, Su Y Q et al . Molecular background of Rh D-positive, D-negative, D^el and weak D phenotypes in Chinese population[J].Vox Sang, 2002;83:156.
  • 10Wagner F F, Frohmajer A, Flegel W A. RHD positive haplotypes in D negative Europeans[J]. BMC Genet, 2001 ;2:10.

共引文献66

同被引文献56

  • 1邵超鹏,苏宇清,熊文,张印则,李桢,周丹.DEL红细胞膜D抗原表位分析[J].国际输血及血液学杂志,2006,29(3):193-195. 被引量：33
  • 2孙国栋,段现民,张彦平,尹志柱,牛小利,李艳凤,赵有良,牛海江.中国人群中发现的主要弱D型——弱D15个体的分子背景研究[J].中国实验血液学杂志,2006,14(5):1024-1028. 被引量：27
  • 3孙国栋,王晓平.Rh血型系统弱D及部分D研究进展[J].临床输血与检验,2007,9(2):183-185. 被引量：28
  • 4Luettringhaus TA, Cho D, Ryang DW, et al. An easy RHD genoty- ping strategy for D- East Asian persons applied to Korean blood do- nors. Transfusion, 2006,46(12) :2128 -2137.
  • 5Liu HC, Eng HL, Yang YF, et al. Aberrant RNA splicing in RHD 7 -9 exons of DEL individuals in Taiwan: a mechanism study. Bio- chim Biophys Acta, 2010 , 1800 (6) :565 - 573.
  • 6Wang XD, Wang BL, Ye SL,et al. Non-invasive foetal RHD genoty- ping via real-time PCR of foetal DNA from Chinese RhD-negative ma- ternal plasma. Eur J Clin Invest, 2009 ,39(7) :607 -617.
  • 7Yan L, Wu J, Zhu F, et al. Molecular basis of D variants in Chinese persons. Transfusion, 2007 , 47 (3) : 471 - 477.
  • 8Wagner FF. RHD PCR of D-Negative Blood Donors. Transfus Med Hemother, 2013 ,40(3) :172 - 181.
  • 9Krog GR, Clausen FB, Berkowicz A, et al. Is current seroIog/c RhD typing of blood donors sufficient for avoiding immunization of recipi- ents? Transfusion, 2011 ,51 ( 11 ) :2278 - 2285.
  • 10Cruz BR, Chiba AK, Moritz E, et al. RHD alleles in Brazilian blood donors with weak D or D-negative phenotypes. Transfus Med, 2012, 22(2) :84 -89.

引证文献8

二级引证文献18

中国实验血液学杂志
2012年 第6期

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