摘要
研究影响佛手瓜SRAP扩增反应的主要因子,建立佛手瓜SRAP反应的优化体系,利用SRAP技术对福建省10个佛手瓜栽培种进行亲缘关系分析.筛选出10对具有较高扩增多态性的引物组合,共扩增出条带114条,其中62条为多态性条带,多态率为54.38%.聚类分析结果表明,当遗传距离为0.20时,可将10个品种分为3个类群;遗传距离为0.35时,10个品种聚为一类,表明10个佛手瓜品种的亲缘关系较近.
The main factors affecting the amplification results in the reaction system of sequence-related amplified polymorphism （SRAP） were investigated. SRAP-PCR reaction system of Seehium edu/e was optimized and SRAP was assessed to analyze the ge- netic relationships among 10 Sechium edule cultivars. A total of 114 DNA bands were amplified by 10 selective primers, 62 of which （54.38%） were polymorphic. Cluster analysis showed that the 10 cultivars were dassified into three duster groups with the genetic similarity of 0.20. All cultivars clustered together when the genetic distance was 0.35, which suggested the closer genetic relation- ship of 10 Sechium edule cultivars.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2012年第6期590-594,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省财政专项-福建省农业科学院科技创新团队建设基金项目资助(STIFY06)
国家大宗蔬菜产业技术体系(CARS-25-G-20)
国家科技支撑项目(2007BAD07B03)
关键词
佛手瓜
亲缘关系
SRAP
聚类分析
Sechium edule Swartz genetic relationship
SRAP
cluster analysis
