摘要
建立了血液中卡马西平的固相萃取/液相色谱-串联质谱(LC-MS/MS)定量检测方法。血液中的卡马西平用固相萃取柱(Bond Elut Certify)提取,采用Waters AtlantisTMdC18(150 mm×3.9 mm,5μm)色谱柱,电喷雾离子源,正离子检测,多反应监测方式进行定量分析,以SKF-525A为内标。结果表明,该方法对卡马西平的检出限为0.1μg/L,卡马西平的质量浓度在100～6 000μg/L范围内线性关系良好(r=0.997 6),日内、日间精密度RSD(n=6)不高于8.6%,血液中卡马西平的回收率为81%～90%。该方法具有良好的灵敏度、重现性、稳定性和专属性,可用于法庭与临床的毒物分析。
A method for the quantitative determination of carbamazepine in blood by solid phase extraction（SPE） combining with LC-MS/MS was developed.The carbamazepine in the blood sample was extracted with SPE and separated with Waters AtlantisTM dC18column（150 mm×3.9mm,5 μm）.The detection of carbamazepine was performed using electrospray ionization source,under positive ion mode and multiple reaction monitorring mode with SKF-525A as the internal standard.The calibration curve was linear in the range of 100-6 000 μg/L for carbamazepine,with a detection limit of 0.1 μg/L.The intra-day RSD（n=6） and the inter-day RSD（n=6） were both no more than 8.6%,and the recoveries were more than 80%.With the advantages of high sensitivity,good repeatability,long-time stability and specificity,this method could be used to detect the carbamazepine in forensic and clinical analytic toxicants．
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第12期1595-1598,共4页
Journal of Instrumental Analysis
