摘要
染料由于具有复杂的化学结构通常难以降解.漆酶粗酶液对终浓度为50mg/L的不同化学结构的染料的脱色研究,可快速对蒽醌类茜素红进行脱色可达100%;杂环类中性红、偶氮类刚果红和三苯基甲烷类结晶紫的脱色效果低于茜素红,测试的3种染料均可在没有介体存在的条件下被漆酶脱色,显示出偏肿革裥菌(Lenzites gibbosa)漆酶对茜素红染料脱色具有较大的应用潜力,进而对废水处理具有更好的应用前景.利用PCR和RACE技术首次从该菌株中获得编码漆酶基因的cDNA及其基因组全长序列,基因组大小为2 106bp.通过比较该漆酶基因的cDNA和基因组DNA的全长序列,发现该基因包含11个外显子和10个内含子.cDNA序列的全长为1 982bp,其中包含一个完整的ORF,长度为1 563bp,编码520个氨基酸.
Dyes are usually difficult to be decolorized due to their complex chemical structures.Chemical different dyes were used in the dye decolorization test.Laccase crude enzyme solution to a final concentration of 50 mg/L of different chemical structure of the dye decolorization,And fast grace quinones Alizarin Red decolorization of up to 100%;Heterocyclic neutral red,Azo kind of Congo red and Triphenylmethane crystal violet Decolorizing effect below alizarin red.The result demonstrated the potential application prospects of the laccase from Lenzites gibbosa dye-containing wastewater treatment.Using PCR and RACE technique,We obtain the cDNA and genomic DNA sequence of the laccase gene from Lenzites gibbosa,Genome size for 2 106 bp.By comparing the laccase cDNA gene and genome DNA sequence length,Found that the gene contains 11 exon and 10 introns.cDNA sequence of full length for 1 982 bp,contains a complete ORF,length for 1 563 bp,code 520 amino acids.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第6期744-750,共7页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金项目(30671700)
中国博士后科学基金项目(20090460866)
关键词
偏肿革裥菌
漆酶基因
染料脱色
结构域
Lenzites gibbosa
laccase gene
dye decolorization
function domain
