摘要
建立了基于分子印迹聚合物(MIPs)为吸附剂的固相萃取技术结合高效液相色谱检测饲料中5种磺胺类药物(SAs)的方法。以磺胺嘧啶为模板,采用紫外光引发合成MIPs,以MIPs作为吸附剂制备固相萃取柱,并对上柱、淋洗和洗脱等萃取条件进行了优化。在优化的条件下,5种SAs的检出限为0.14～0.23 mg/kg;定量限为0.38～0.47 mg/kg;平均回收率在72.1%～89.3%之间;批内与批间相对标准偏差分别小于7.9%和9.2%。与碱性氧化铝柱净化相比,分子印迹固相萃取柱净化后杂质更少,选择性更好,方法的定量限更低。
A method was developed for monitoring five sulfonamides（SAs） in feeds by using molecularly imprinted polymers（MIPs） as selective solid-phase extraction sorbents coupled with high performance liquid chromatography.The MIPs were firstly synthesized by ultraviolet initiated using sulfadiazine as template.Then,the molecularly printed solid phase extraction（MISPE） column was prepared,and the MIPs were used as adsorbent.Meanwhile,the procedures of loading,washing and eluting conditions were optimized.Under the optimum conditions,the detection limit range of five SAs was 0.14-0.23 mg/kg and the quantification limit range was 0.38-0.47 mg/kg.The average recoveries were in the range of 72.1%-89.3% with a batch and batch sampling relative standard deviations less than 7.9% and 9.2%,respectively.It showed that the MISPE column decreased the matrix effect and had a better selectivity and a lower limit of quantification than Alumina B column.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2012年第12期1871-1876,共6页
Chinese Journal of Analytical Chemistry
基金
食品科学与技术国家重点实验室自由探索课题(No.SKLF-TS-200815)资助
关键词
分子印迹聚合物
固相萃取
磺胺类药物
饲料
高效液相色谱
Molecularly imprinted polymer
Solid-phase extraction
Sulfonamides
Feed
High performance liquid chromatography
