摘要
为了探讨蒲公英糖蛋白(TMGP)体内外抗氧化活性,采用H2O2、Fe2+、DPPH.和总抗氧化能力反应体系,检测蒲公英糖蛋白体外抗氧化活性,并与VC进行比较;同时建立D-半乳糖所致衰老小鼠模型,测定并比较TMGP组与模型组的血清、肝脏和脑内MDA含量和SOD、CAT、GSH-Px活性。结果显示:(1)随着TMGP浓度增加,其Fe2+、H2O2和DPPH.清除率均有所增加,当TMGP浓度为1.72mg/mL时,对H2O2、Fe2+清除率分别达到76.8%和55%,接近VC水平;当TMGP浓度为1.5mg/mL时,对DPPH.的清除率达到77%,也与VC水平相当,说明TMGP具有一定的体外总体抗氧化能力。(2)将TMGP按低、中、高剂量分别给小白鼠灌胃,可显著增强小鼠体内血清、肝脏、脑组织中SOD、CAT、GSH-Px活性,降低MDA含量,从而提高衰老小白鼠体内的抗氧化能力。研究表明,TMGP具有明显的体内外抗氧化作用。
Abstract：We investigated the antioxidant effect of Taraxacum mongolicum glycoprotein （TMGP） in vitro and invivo. The reaction system of H2O2,Fe2＋,DPPH · and total antioxidant capacity was used to detect the antioxidant activity of TMGP in vitro and was compared with Vc. The contents of MDA and the activi- ties of SOD,CAT,GSH-Px in plasma, liver and brain were respectively measured in aged mice model in- duced by D-galactose. The result showed that. （1） The scavenging capacity of TMGP for H2O2 and Fe2＋ were enhanced as TMGP concentration increased. The H202,Fe2＋ scavenging rates reached 76. 8% and 55% respectively at 1.72 mg/mL TMGP,close to the level of Vc ;When TMGP concentration at 1.5 mg/ mL,the DPPH · scavenging rate reached 77% and it was near Vc;（2）The animal experiment showed that the content of MDA decreased and the SOD,CAT,GSH-Px activities increased in plasma, liver and brain in all TMGP groups,significantly increased the antioxidant capacity of mice. TMGP has obvious antioxidant effect in vitro and in vivo.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第12期2486-2491,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省自然科学基础研究计划项目(2006B18)
关键词
蒲公英
糖蛋白
自由基
抗氧化
Taraxacum mongolicum
glycoprotein
free radical
antioxidant
