摘要
采用半静态实验方法,研究了低(32μg.L-1)、中(160μg.L-1)和高(800μg.L-1)3个浓度BDE-209对菲律宾蛤仔外套膜抗氧化酶(SOD、CAT及POD)活性和GSH、MDA含量的影响。结果表明:BDE-209对菲律宾蛤仔外套膜SOD、CAT和POD活性均呈现先促进后抑制的作用;低浓度组SOD活性在暴露第2天达到最高,为56.61U.mg-1prot,显著高于对照组(P<0.05);清水恢复7d后,各浓度组SOD活性与对照组没有显著差异;暴露2～15d,各浓度组CAT活性均显著高于对照组(P<0.05),中高浓度组CAT活性均在暴露第7天达到最高诱导率;中浓度组POD活性在暴露第1天即达到最高,是空白对照的1.33倍,高浓度组POD活性在清水恢复期间均受到显著抑制作用(P<0.05);随着BDE-209暴露时间的延长,菲律宾蛤仔外套膜MDA含量呈现先下降后上升趋势,清水恢复3d后各浓度组MDA含量与对照组没有显著差异;BDE-209对菲律宾蛤仔外套膜GSH含量则呈现先诱导后抑制的作用,其中暴露第3天各浓度组GSH含量均达到最低(P<0.05)。BDE-209可诱导菲律宾蛤仔氧化应激反应,SOD、CAT和POD在自由基清除中发挥重要作用,GSH含量可作为BDE-209对菲律宾蛤仔胁迫的敏感指标。
A semi-static experiment was conducted to study the ettects ol dlllerent concentrations （low, 32 μg . L-1 ; medium, 160 μg . L-l ; and high, 800 μg . L-1 ） decabromodiphenyl ether （BDE-209） on the SOD, CAT and POD activities and GSH and MDA contents of RUditapes philippinarum mantle membrane. Under the effects of BDE-209, the SOD, CAT, and POD activities decreased after an initial increase. After 2 days exposure to low concentration BDE-209, the SOD activity reached the maximum （56.61 mg-t prot）, being significantly higher than the control. No significant difference was observed in the SOD activity between the control and the expo-sure groups after 7 days recovery in distilled water. After exposure to different concentrations BDE-209 for 2-15 days, the CAT activity was significantly higher than the control, being the highest on the 7th day when exposed to medium and high concentrations BDE-209. The POD activity reached its maximum on the 1st day exposed to medium concentration BDE-209, being 1.33 times of the control, while the POD activity exposed to high concentration BDE-209 was constantly significantly lower till the end of the experiment. With the increasing time of BDE-209 exposure, the MDA content increased after an initial decrease, and had less difference with the control after 3 days recovery in distilled water. The variation trend of GSH was contrary to that of MDA. The GSH content increased significantly at the beginning of the experiment, but decreased with time elapsed, being the lowest at the 3rd day of exposure （P〈0.05）. The results of this study indicated that BDE-209 could induce the oxidative stress response of R. philippinarum, the CAT, SOD, and POD played an important role in removing oxygen fi＇ee radical, and the GSH could be regard as a sensitive index for BDE-209 stress.
出处
《生态学杂志》
CAS
CSCD
北大核心
2013年第1期122-128,共7页
Chinese Journal of Ecology
基金
广东省自然基金项目(S2011040005251)
广东省科技计划项目(2009B030600001)
中央级公益性科研院所基本科研业务费专项资金项目(2012TS14)
中国水产科学研究院基本科研业务费专项课题(2012A0203)资助
关键词
持久性有机物
氧化应激
超氧化物歧化酶
过氧化物酶
persistent organic pollutant
oxidative stress response
superoxide dismutase
perox-idase.
