摘要
目的研究麝香酮含药血清对骨髓间充质干细胞(BMSCs)的增殖及其向成骨细胞分化的影响,初步了解麝香酮单体对骨髓间充质干细胞的作用机制。方法贴壁筛选法提取大鼠BMSCs,培养至第3代,流式细胞仪检测细胞表面抗原,成骨成脂诱导,鉴定认为培养成功后,利用制备的不同浓度麝香酮含药血清干预。测定细胞增殖率、碱性磷酸酶(ALP)活性、钙盐沉积量、骨钙素分泌量等,研究麝香酮含药血清对大鼠干细胞增殖、分化的影响。结果筛选纯化的BMSCs呈均一的成纤维细胞样,贴壁生长,以长梭形为主;表型鉴定:CD45阴性,CD44、CD95均呈阳性表达;用含药血清干预后的增殖率、ALP活性、钙盐沉积量、骨钙素分泌量、钙化结节量均高于空白对照组。结论麝香酮含药血清可以促进BMSCs的增殖、成骨分化。
Objective To study the effects of muscone-containing serum on proliferation and osteogenic differentiation of mesenchymal stem cells （BMSCs） to understand the action mechanism of muscone monomer on mesenchymal stem cells. Methods Adherence screening was used to extract rat BMSCs, which were then cultured to the third generation. BMSC surface antigen as well as osteogenic and adipogenic induction were detected by flow cytometry. Then different concentrations of muscone-containing serum were used for intervention when successful culture was identified. Cell proliferation rate, ALP activity, calcium deposition, and osteocalcin secretion were determined to study the effects of muscone-containing serum on the proliferation and differentiation of rat stem cells. Results Screened purified BMSCs showed homogeneous fibroblast-like, adherent growth, mainly with long fusiform. CD45 was negative while CD44 and CD90 were positive. The proliferation rate, ALP activity, calcium deposition, osteocalcin secretion, and calcified nodules were higher in muscone-containing serum intervention group than in the blank control group. Conclusion Muscone-containing serum can promote the proliferation and osteogenic differentiation of BMSCs.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期110-114,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.81060299)~~
关键词
骨髓间充质干细胞
增殖
分化
麝香酮
含药血清
mesenchymal stem cell （BMSC）
proliferation
differentiation
muscone
drug-containing serum
