摘要
以丽格海棠巴科斯品系叶片为材料,进行外植体的诱导培养、不定芽继代增殖培养、无根苗生根培养等组织培养技术研究,探索其适宜的培养基和培养条件.结果表明:在MS+BA 1.0 mg/L+NAA 0.2 mg/L培养基上丽格海棠叶片诱导不定芽效果最好;在MS+BA 0.1 mg/L+NAA 0.01 mg/L培养基上继代增殖效果较好;在1/2 MS+NAA 0.2 mg/L培养基生根诱导培养效果较好.苗高6 cm可出瓶过渡移栽,并保持90%以上的空气湿度,温度为(25±3)℃,成活率最高.
We take the leaves of Rigghedan Ba Kos strains as materials to conduct tissue culture technology research, including induction culture explanting subculture to adventitious buds, rooting culture to rootless seedling, in order to explore the optimal culture medium and culture condition. The results show that the best effect of induction adventitious buds on Rieger begonias leaves occurred in culture medium as MS ＋ BA 1.0 mg/L ＋ NAA 0. 2 mg/L medium; subculture proliferation effect is better in culture medium as MS ＋ BA 0. 1 mg/L ＋ NAA 0. 01 mg/L; rooting induction training effect is better in culture medium as 1/2 MS ＋ NAA 0. 2 mg/L. As seedling height reaches 6 cm, transplant could be carried. After transplanting, highest survival rate could realize under the condition as maintaining more than 90% air humidity, temperature （25 ± 3） ℃.
出处
《昆明学院学报》
2012年第6期57-59,共3页
Journal of Kunming University
关键词
丽格海棠
组织培养
快速繁殖
外植体
Rieger begonia
tissue culture
rapid propagation
explants
