摘要
目的:建立高效液相色谱法同时测定利胆排毒口服液中绿原酸、栀子苷、丹酚酸B含量的方法。方法:采用AtlantisT3柱(150 mm×4.6 mm,3μm),0.05%磷酸溶液为流动相A,乙腈为流动相B,梯度洗脱,流速为0.8 ml·min^(-1),检测波长为254 nm,柱温为30℃。结果:绿原酸、栀子苷、丹酚酸B分离度好,绿原酸在6.32～306.60μg·ml^(-1)的范围内有良好的线性关系(r=0.999 1),平均回收率为98.72%,RSD为1.30%(n=6);栀子苷在12.04～602.00μg·ml^(-1)范围内有良好线性关系(r=0 999 8),平均回收率为99.43%,RSD为0.80%(n=6);丹酚酸B在2.67～133.40μg·ml^(-1)范围内有良好线性关系(r=0.999 5),平均回收率为95.08%,RSD为1.98%(n=6)。结论:本方法可同时测定绿原酸、栀子苷、丹酚酸B的含量,准确度高,重复性好,可作为利胆排毒口服液的含量测定方法。
Objective：To establish an HPLC method for the simultaneous determination of chlorogenic acid,geniposide and salvianolic acid B in Lidan Paidu oral liquids.Method：The samples were analyzed on anAtlantis T3（150 mm x4.6 mm,3μm） column with the mobile phase A of 0.05%phosphoric acid solution and mobile phase B of acetonitrile with gradient elution.The flow rate was 0.8 ml ？ min,the detection wavelength was 254 nm and the column temperature was 30t.Result;The peaks of the three ingredients were separated effectively without interference.The linear range of chlorogenic acid was 6.32-306.60μg ？ ml^（-1）（r =0.999 1 ） and the average recovery was 98.72%（RSD = 1.30%,n =6）.The linear range of geniposide was 12.04-602.00μg ？ ml^（-1）（r =0.999 8 ） and the average recovery was 99.43%（RSD =0.80%,n =6）.The linear range of salvianolic acid was 2.67-133.40μg ？ ml^（-1）（r = 0.999 5） and the average recovery was 95.08%（RSD = 1.98%,n =6）.Conclusion：The method is accurate and reproducible,and can be used in the quality control of Lidan Paidu oral liquids.
出处
《中国药师》
CAS
2012年第11期1562-1564,共3页
China Pharmacist
