摘要
目的研究收涩中药乌梅、椿皮、金樱子、五味子、五倍子、赤石脂、禹余粮对鸡胚尿囊膜生成的影响。方法采用血清药理学方法,制备收涩药物的大鼠血清;建立鸡胚绒毛尿囊膜模型,并应用鸡胚绒毛尿囊膜模型进行活性筛选。于鸡胚发育的第5天,将加药物血清后的明胶海绵载体加于鸡胚绒毛尿囊膜上,孵育48 h后,固定鸡胚绒毛尿囊膜,取出,采用医用图像采集系统分析结果。结果收涩药物金樱子、五味子、五倍子、乌梅、赤石脂、椿皮对鸡胚绒毛尿囊膜新生血管数目有一定抑制作用(P<0.05);乌梅组、椿皮组对抑制新血管生成有一定的趋势,与空白对照组比较,差异无统计学意义(P>0.05)。结论收涩中药对鸡胚绒毛尿囊膜新生血管有一定的抑制作用。
Objective To study the impacts of astringent Chinese herbs such as fructus mume,cortex ailanthi, fructus rosae laevigatae, schisandra chinensis, galla chinensis, halloysitum rubrum and limonitum on angiogenesis of chick embryo ehorioallantoic membrane （CAM）. Methods The serum pharmacological method was adopted to prepare the rat serum with astringent herbs involved. The model of CAM was set up and used for the activity screening. On the 5th day of chick embryo development, the gelatin sponge carrier with drug serum added was coated on CAM. After incubation for 48 h, it was fixed on CAM. The medical im- age collection system was adopted for the result analysis. Results The astringent Chinese herbs such as fruc- tus rosae laevigatae, schisandra chinensis, galla chinensis, fructus mume, halloysitum rubrum and cortex ailan- thi inhibited the numbers of CAM to a certain extent（ P 〈 0.05 ）. The results in fructus mume group and cor- tex ailanthic group presented a certain tendency of angiogenesis inhibition as compared with the blank control group, but without indicating the statistical significant difference （P 〉 0. 05 ）. Conclusion The astringent Chinese herbs present a certain inhibition on CAM angiogenesis.
出处
《世界中西医结合杂志》
2012年第12期1042-1044,共3页
World Journal of Integrated Traditional and Western Medicine
基金
山西省科学技术厅攻关项目(No.20090311053-3)
关键词
收涩
中药
鸡胚尿囊膜
血管生成
Astringent
Chinese Medicine
Chick Embryo Chorioallantoic Membrane（CAM）
Anglo- genesis
