摘要
在离体条件下,以我国主栽香蕉品种‘巴西蕉’(Musa AAA Cavendish)的多芽体和不定芽为材料,采用EMS和NaN3对其进行诱变处理,以镰刀菌酸毒素为选择压,筛选抗镰刀菌酸的材料。结果表明,EMS和NaN3诱变处理多芽体适宜的浓度和时间组合分别为1.6%+3h和2g/L+3h,NaN3诱变处理不定芽适宜的浓度和时间组合为2g/L+1h。镰刀菌酸毒素筛选多芽体和不定芽适宜浓度范围分别为36～45mg/L和10～20mg/L,筛选获得的多芽体和不定芽多次继代培养后仍保持对镰刀菌酸的抗性。本研究可为香蕉抗枯萎病育种提供有价值的材料和技术参考。
Fusarium wilt is a devastating disease for banana planting. This work was initiated to develop an in vitro technique suitable for mutation induction on ＇ Baxijiao＇ （Musa AAA Cavendish） using chemical mutagens. The experiment was to established the optimal dose of chemical mutagenesis for mutagenic induction of the banana cv. ＇Baxijiao＇ for subsequent mutation breeding. Scalps and shoot apices of in vitro-grown cultures of banana cv. ＇Baxijiao＇ were treated with various concentrations of the mutagens ethylmethanesulfonate （EMS）, sodium azide （ NaN3 ） and fusaric acid to evaluate their effectiveness in inducing mutations and to select variants tolerant to the fungus Fusarium oxysporum f. sp. cubense. The results showed that the optimal concentration and treatments period of EMS and NaN3 on the scalps was 1.6% ＋ 3h and 2g/L ＋ 3h, respectively, but on the shoot apices from adventitious buds, the optimal concentration and treatments period of NaN3 was 2g/L ＋ 1 h. The suitable concentration range of fusaric acid on the scalps and adventitious buds was 36 - g5mg/L and 10 -20mg/L, respectively, for mutation screening. The selected scalps and adventitious buds still kept resistance to fusaric acid after subcultures. However, further identification test is needed for a definitive and reliable result. This study provides a valuable data for the breeding of banana resistance to fusarium wilt.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第9期1237-1243,共7页
Journal of Nuclear Agricultural Sciences
基金
国家科技部863项目(2011AA10020603)
国家自然科学基金项目(U1131004)
国家香蕉产业技术体系建设项目(CARS-32-04B)
国家科技部国际合作项目(2010DFA31760)
国家农业部948项目(2011-G16)
广东省现代农业产业技术体系专项(lnsgtx-03)
关键词
'巴西蕉’
多芽体
不定芽
EMS
NAN3
＇ Baxijiao＇
scalps
adventitious buds
ethylmethanesulfonate
sodium azide
