摘要
目的:探讨口服透明质酸(HA)对小鼠佐剂性关节炎(AA)的治疗作用。方法:将40只6周龄的雄性小鼠随机分成4个小组,正常组、模型组、HA组和双氯芬酸钠组。通过给模型组、HA组和双氯芬酸钠组小鼠右后足注射弗氏完全佐剂(FCA)建立佐剂性关节炎模型,经连续4周口服透明质酸进行治疗后,分别对小鼠的体质量、足跖厚度进行测量,观察小鼠膝关节肿胀程度并评分,同时采用酶联免疫吸附实验(ELISA)检测小鼠血清中白介素-1(IL-1)和HA的含量。结果:口服HA能显著降低小鼠足跖厚度和关节炎指数,同时血清中IL-1和HA含量亦显著降低;并且各项指标与双氯芬酸钠无显著性差异。结论:口服透明质酸能有效地缓解小鼠膝关节的肿胀,降低血清中IL-1的含量,增强活动能力,因而对小鼠佐剂性关节炎具有良好的治疗作用。
Objective： To examine the therapeutic effect of oral administration of hyaluronic acid（HA） on adjuvant arthritis（AA） in mice.Methods： Forty 6-weeks-old healthy mice were randomly divided into 4 groups： normal,model,HA and diclofenac groups.AA mouse models were created by injection of Fluoride’s complete adjuvant（FCA） into the right hind paw.After 4 successive weeks of oral HA administration,mouse body weight and footpad thickness were measured and knee swelling was observed and scored.In addition,serum levels of interleukin-1（IL-1） and HA were assayed.Results： Oral HA administration resulted in a significant reduction in mouse footpad thickness and arthritis index and serum levels of IL-1 and HA and showed no significant differences in various indicators compared with diclofenac.Conclusions： Oral HA administration is effective at alleviating knee swelling,lowering serum interleukin-1 level and enhancing physical activities in AA mice and thus has a powerful therapeutic effect on adjuvant arthritis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第23期287-290,共4页
Food Science
基金
上海海洋大学-日本丘比株式会社合作研究基金项目
关键词
口服透明质酸
动物模型
佐剂性关节炎
oral administration of hyaluronic acid
animal model
adjuvant arthritis
