摘要
目的 观察补锌对急性冷应激大鼠 ACTH分泌及小鼠耐寒力的影响 .方法 雄性 SD大鼠 32只 ,随机分为 4组 ,1,3组给予正常饲料 ,饮自来水 ,2 ,4组给予正常饲料 ,饮高锌自来水 (补锌 1mg· kg- 1 · d- 1 ) ,5 d后 ,3,4组于 - 15℃冷暴 6 0 min后与 1,2组一同宰杀 ,测血浆 ACTH,血清、肝脏和骨骼肌锌含量 .雄性昆明种小白鼠 40只 ,随机分为 2组 ,1组正常进水进食 ,2组饮高锌水 (补锌 1mg· kg- 1·d- 1 ) ,5 d后 ,两组小鼠同时暴露于 - 15℃的冷室中 ,记录死亡时间 .结果 补锌可以使正常大鼠血浆 ACTH水平升高 (P<0 .0 5 ) ,- 15℃冷暴 6 0 min可使大鼠 ACTH水平明显升高(P<0 .0 1) ,补锌可以降低其升高的幅度 .补锌使正常大鼠肝和骨骼肌锌含量升高 ,并减轻冷暴大鼠肝和骨骼肌的锌含量降低的程度 .补锌组小鼠在 - 15℃冷室内的存活时间比未补锌组延长 15 .9% (P<0 .0 5 ) .
AIM To study the effects of zinc on ACTH secre tion and cold endurance in animals. METHODS Thirty two male SD rats were randomized into 4 groups. Rats of group I and III drank tap water, group II and IV drank high zinc water (1 mg·kg -1 ·d -1 ), respectively, and 5 days later, rats from Group III and IV underwent exposure to cold at -15℃ for 60 min. All rats were sacrificed for the detection of plasma ACTH and the level of zinc in the serum and tissues. Forty male Kunming mice were randomized into 2 groups. Mice of Group I and II drank tap water and high zinc water (1 mg·kg -1 ·d -1 ), respectively, and 5 days later, all mice underwent exposure to cold at -15℃, and the survival time was recorded. RESULTS The ACTH concentration in plasma of group 2 increased significantly than that of the control ( P <0.05). Acute cold exposure led to a marked increase in ACTH concentration in plasma of rats and high zinc intake inhibited the increase to a certain extent. High zinc intake increased the zinc concentration in tissues in normal rats, but it restrained the decrease of zinc concentration in tissues due to acute cold exposure. The survival time of high zinc intake mice in cold condition (-15℃) was 15.9% longer than that of the control group. CONCLUSION Zinc might play an important role in cold stress.
出处
《第四军医大学学报》
2000年第6期699-701,共3页
Journal of the Fourth Military Medical University
基金
全军青年基金!( 96Q0 81)
