摘要
以109只海南文昌鸡为试验动物,利用PCR-SSCP技术检测了促卵泡激素(follicle-stimulating hormoneβ,FSHβ)基因的多态性,在其内含子1区域内发现了1个SNP,在群体中检测出2种基因型:AA、AB。将不同基因型个体与产蛋数、畸形蛋数、体重和开产日龄等进行了关联分析。结果表明,AA基因型鸡个体的产蛋数比AB基因型个体多9.54枚,畸形蛋数比AB基因型个体少1.54枚,开产日龄比AB基因型个体早2.97 d,且差异均不显著(P>0.05)。22～26、27～30和31～34周龄的AA基因型鸡的产蛋量显著高于AB基因型(P<0.05),而18～21和35～39周龄的AA基因型鸡的产蛋量也明显高于AB基因型,但差异不显著(P>0.05)。AA基因型鸡6、8、14、20周龄的体重高于AB基因型个体,AA基因型个体18周龄的体重却低于AB基因型个体,差异均不显著(P>0.05)。总体而言,FSHβ基因位点的A等位基因对产蛋数、畸形蛋数、体重和开产日龄的影响均未达到差异显著水平。
This study detected gene polymorphisms of follicle-stimulating hormone β（FSHβ）,in which 1 SNP in intron 1 region was found,and 2 genotypes were detected,respectively,for genotype AA and AB by means of PCR-SSCP technique with 109 Wenchang chickens in Hainan as experimental animals.To conduct correlation analysis among different genotypes and egg production（NE）,the number of abnormal eggs（ENA）,body weight（BW）,and age at first egg（AFG）,the results showed that NE of AA genotype chicken individual was more 9.54 than that of AB genotype;ENA of AA genotype was less 1.54 than that of AB genotype;AFG of AA genotype was 2.97 days earlier than that of AB genotype,and the difference was not significantly（P0.05）.At 22 to 26,27 to 30 and 31 to 34 weeks of age,the egg production of AA genotype chicken was obviously higher than that of the AB genotype,and the difference was significantly（P 0.05）.At 18 to 21 and 35 to 39 weeks of age,the egg production of AA genotype chicken also was obviously higher than that of the AB genotype,but the difference was not significantly（P0.05）.BW of AA genotype was higher than that of AB genotype at 6,8,14,20 weeks of age,but BW of AA genotype was higher than that of AB genotype at 18 weeks of age,both of the differences were not significantly（P0.05）.In general,A allele of the FSHβ gene did not significantly impact on NE,ENA,BW and AFG.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第12期117-121,共5页
China Animal Husbandry & Veterinary Medicine
基金
山东省农业良种工程项目(2011LZ15-4)
