摘要
目的探讨荧光原位杂交(FISH)技术检测人类宫颈端粒酶RNA组分(hTERC)基因在不同程度宫颈病变中的表达及其临床意义。方法收集133例妇科疾病患者的宫颈脱落细胞及组织,以及20位健康体检妇女的正常宫颈脱落细胞,应用FISH技术检测宫颈脱落细胞以及病变组织中的hTERC基因扩增情况。结果单纯炎症病变、未明确诊断的不典型鳞状细胞(ASCUS)、低度鳞状上皮内病变(LSIL)、高度鳞状上皮内病变(HSIL)及鳞状细胞癌患者宫颈脱落细胞hTERC基因扩增的阳性率分别为0.00%、15.79%、81.82%、95.45%及100.00%;单纯炎症或宫颈上皮内瘤变(CIN)Ⅰ级、CINⅡ级、CINⅢ级及宫颈浸润癌患者病变组织hTERC基因扩增的阳性率分别为3.45%、52.38%、71.43%及100.00%。宫颈癌患者宫颈脱落细胞及病变组织hTERC基因的表达与CIN的差异有统计学意义(P<0.05)。结论 hTERC基因扩增可预测宫颈病变的发展趋势。
Objective To explore the detection of human telomerase RNA component（hTERC） gene expressed in different levels of cervical lesions by fluorescence in situ hybridization（FISH） and its clinical significance. Methods Cervical exfoliated cells and tissues of 133 patients with gynecological disease and Z0 healthy woman who accepted physical examination were collected. FISH was employed to detect the amplification of hTERC gene in cervical exfoliated cells and lesion tissues. Results Positive rates of hTERC gene amplification in cervical exfoliated cells of patients with simplex inflammatory lesions, atypical squamous cells of unde- termined significance（ASCUS）, low-grade squamous intraepithelial lesion（LSIL）, high-grade squamous intraepithelial lesion（HSIL） and squamous cell carcinoma were 0.00%,15.79%/0,81.82%,95.45% and 100.00%,respectively,while those in lesion tissues of patients with simplex inflammatory lesions or cervical intraepithelial neoplasia（CIN） Ⅰ , CIN Ⅱ , CIN Ⅲ and invasive cervical carcino- ma were 3.45 %, 52.38 %, 71.43 % and 100.00 %, respectively. Difference of hTERC gene expression in cervical exfoliated cells and lesion tissues between cervical carcinoma and CIN was not statistically significant（P〈0.05）. Conclusion hTERC gene amplification may predict the development trend of cervical lesions.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第1期13-15,18,共4页
Chongqing medicine
基金
广西医疗卫生科研课题(桂卫Z2008079)
关键词
原位杂交
荧光
端粒
末端转移酶
宫颈上皮内瘤样病变
in situ hybridization,fluorescence
telomerase
cervical intraepithelial neoplasia
