摘要
采用两步法冷冻技术超低温保存盐生杜氏藻 (Dunaliellasalina (Dunal)Teod .)和青岛大扁藻 (Platymonashel golandicaKylinvar.tsingtaoensis)。当二甲基亚砜 (DMSO)浓度分别为 5 %和 2 0 % ,平衡时间均为 30min ;预冻温度及保持时间分别为 - 40℃ ,6 0min和 - 30℃ ,30min ;化冻后分别在 0℃和室温下采用慢速稀释法去除DMSO时 ,盐生杜氏藻和青岛大扁藻都达到了最高存活率 ,分别为 6 8 5 %和 78 3%。
WT5BX]Dunaliella salina (Dunal) Teod. and Platymonas helgolandica Kylin var. tsingtaoensis were cryopreserved in liquid nitrogen with a two_step cryopreservation method. With 5% and 20% dimethylsulphoxyde (DMSO) concentration respectively through 30 min equilibrium time, and under -40 ℃, 60 min and -30 ℃, 30 min respectively as prefreezing temperature and sustained time, and by using slow dilution method to remove DMSO from the sample at 0 ℃ and room temperature respectively after sample thawing, D. salina and P. helgolandica var. tsingtaoensis gained the highest survival rates that were 68.5% and 78.3% respectively.
基金
国家自然科学基金!资助项目 ( 3 9470 0 65 )&&
关键词
盐生杜氏藻
青岛大扁藻
超低温保存
饵料
DMSO
Dunaliella salina
Platymonas helgolandica
cryopreservation (liquid nitrogen)
