摘要
目的:研究Sox9基因在软骨组织表达异常是否与马蹄内翻足发病相关。方法:制作先天性马蹄内翻足(idiopathiccongenital talipes equinovarus,ICTEV)大鼠模型,取其足踝软骨组织,用RT-PCR及免疫组化方法对Sox9基因表达量和部位与对照组进行对比分析。结果:Sox9基因在ICTEV大鼠模型足踝软骨组织的表达无论是在mRNA还是蛋白水平均比对照组高。结论:Sox9基因在ICTEV大鼠模型足踝的软骨组织表达升高可能与马蹄内翻足发病相关。
Objective： To study the relation between the expression level of Sox9 gene in cartilage tissue and pathogenesis of idio- pathic congenital talipes equinovarus （ICTEV）. Method： Sox9 gene expression amount and location were analyzed by RT-PCR and immunohistochemical staining in ankle cartilage tissue of ICTEV rat model and the control group. Results： The expression level of Sox9 gene in ankle cartilage tissue of ICTEV rat model both at the mRNA or protein levels were higher than those of the control. Conclusion： The expression of Sox9 genes may be related with pathogenesis of ICTEV.
出处
《沈阳医学院学报》
2012年第4期199-201,共3页
Journal of Shenyang Medical College
基金
沈阳医学院科学研究基金(No.20091001)
关键词
SOX9基因
马蹄内翻足
软骨组织
表达
Sox9 gene
idiopathic congenital talipes equinovarus
cartilage tissue
expression
