摘要
运用分子生物学技术对微生物来源的酶进行分子改性和人工进化在过去几年中取得了令人瞩目的进展。本文综述了用于酶分子改性和人工进化的主要分子生物学方法 ,如易错PCR技术。
There exists a remarkably progress in molecular modification and artificial evolution of microbial enzymes in recent years.Error prone polymerase chain reaction and DNA shuffling have been developed and successfully applied and a lot of startling achievements have been obtained in artificial evolution of enzymes.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第3期301-303,共3页
Chinese Journal of Biotechnology