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北京地区2006-2010年女性HIV-1感染者流行毒株分子特征研究 被引量:1

Molecular-epidemiological characteristics of HIV-1 isolated from newly diagnosed female subjects in Beijing, 2006-2010
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摘要 目的分析北京地区女性HIV-I感染者流行毒株分子特征。方法随机采集2006—2010年北京地区新发现女性HIul感染者的抗凝全血标本100份,分离血浆,提取病毒RNA,用反转录/巢式聚合酶链反应扩增病毒gag基因,并进行序列测定和亚型分析。结果系统进化分析确定北京地区女性HIV-1感染者存在Al(1)、A2(1)、B(3)、B’(23)、C(8)、G(2)、H(1)、CRF01_AE(18)、CRF02AG(3)、CRF06cpx(1)、CRF07BC(14)、CRF08BC(4)和B’/C(3)13种亚型,其比例分别为1.22%、1.22%、3.66%、28.05%、9.76%、2.44%、1.22%、21.95%、3.66%、1.22%、17.07%、4.88%和3.66%,B’、CRF01AE和CRF07BC为主要亚型,6种少见亚型(A1、A2、G、H、CRF02AG和CRF06cpx)合计占11.0%。结论北京地区女性HIV-1感染者流行毒株遗传多样性高于男性,B’亚型比例高,少见亚型比例也较高。 Objective To analyze the molecular-epidemiological characteristics of HIV-1 strains prevailing among female people living with HIV in Beijing. Methods Gag gene fragments from the 100 newly diagnosed female HIV-1 infections during 2006 to 2010 in Beijing were amplified, sequenced, and phylogenetically analyzed. Results Eighty-two HIV-1 gag gene fragments were amplified and analyzed. 1 (1.22%), 1 (1.22%), 3(3.66%) ,23 (28.05%), 8(9.76%) ,2(2.44%), 1 ( 1.22% ), 18 (21.95% ), 3 (3 66% ), 1 ( 1.22% ), 14 ( 17.07% ), 4 (4.88%) and 3 (3.66%) individuals were infected with HIV-I subtypes A1, A2, B, B', C, D, G, H, CRF0 I_AE, CRF02_AG, CRF07 BC, CRF08_BC and B'/C recombinants respectively. Conclusion The subtypes circulating in females HIV infections in Beijing were more diversities than in males and the proportions orB' and rare subtypes were relatively high. Surveillance programs on HIV-1 genetic diversity should be strengthened.
出处 《中华流行病学杂志》 CAS CSCD 北大核心 2013年第1期49-52,共4页 Chinese Journal of Epidemiology
基金 基金项目:国家科技重大专项(2012ZXl0001-005)
关键词 艾滋病病毒 亚型 巢式聚合酶链反应 Human immunodeficiency virus Subtype Nested-PCR
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