摘要
目的:研究大黄饮片经60Co-γ射线辐照前后5种主要有效成分的含量变化。方法:取经60Co-γ射线常规剂量辐照前后的大黄饮片,采用高效液相色谱法测定其中5种主要有效成分(芦荟大黄素、大黄素、大黄酸、大黄酚、大黄素甲醚)的含量。色谱柱为SPHERIGEL ODS C18(150mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水(80∶20,V/V),检测波长为254nm。结果:大黄饮片中大黄素的含量最高,其次是大黄素甲醚、大黄酸和大黄酚,含量最低的是芦荟大黄素。大黄饮片在经过辐照以后,各组分含量都较原药材含量有所下降,其中以大黄素甲醚损失最大,而芦荟大黄素损失较少。在放置6个月后,未辐照的大黄饮片中主要有效成分的含量均有不同程度下降;而经60Co-γ射线辐照后的大黄饮片,其主要有效成分含量虽有所降低,但是其成分稳定性增加。结论:在对药材或制剂进行灭菌处理时应考虑到射线对化学成分的影响,灭菌后的制剂应重新对其检测指标进行定量分析,以确保指标含量在国家标准范围内。
OBJECTIVE：To study the content change for 5 active components in Rhei Radix et Rhizoma decoction pieces before and after the irradiation by 60↑Co-γray.METHODS：Rhei Radix et Rhizoma decoction pieces were collected after the irradiation by normal dose of 60↑Co-γray.The contents of 5 main active components were determined by HPLC method（aloe-emodin,emodin,rhein,chrysophanol,physcion）.The determination was performed on SPHERIGEL ODS C 18（150 mm×4.6 mm,5 μm）with mobile phase consisted of methanol-0.1% phosphoric acid（80：20,V/V）.The detection wavelength was set at 254 nm.RESULTS：The content of emodin in Rhei Radix et Rhizoma decoction pieces was the highest;followed by physcion and chrysophanol;the contents of aloe-emodin was the lowest.After irradiation,the contents of each component were lower than before,especially physcion;while less that of aloe-emodin.After half year,the main active components of un-irradiated Rhei Radix et Rhizoma decoction pieces decreased to different extents;the contents of main components in Rhei Radix et Rhizoma decoction pieces decreased to some extents after irradiation by 60↑Co-γray,and stability of the components increased.CONCLUSION：The effects of ray on chemical components should be considered during medicinal material or preparation sterilization.The index of preparations should be re-detected and analyzed quantitatively after sterilization to guarantee the contents of active components within national standard.
出处
《中国药房》
CAS
CSCD
2013年第3期254-256,共3页
China Pharmacy
