摘要
流式细胞术(flow cytometry,FCM)是一种单细胞快速定量分析技术,为精子功能研究提供了一种高通量、多参数的检测手段。FCM在外用避孕药对精子功能评估中的应用,主要包括精子质膜完整性、DNA完整性、顶体反应、线粒体功能、氧化应激和精子凋亡等方面。表明FCM是精子功能研究的有效工具,有助于阐明新型外用避孕药的药效和作用机制。
Flow cytometry （FCM） is a quantitative technology employed in cell counting, sorting, biomarker detection and protein engineering. It allows simultaneous multiparametric analysis of the sperm function. In this review we introduced the application of FCM in the evaluation of topical contraceptives, including plasma mem- brane integrity, DNA intactness, changes induced during capacitation, mitochondrial status, oxidative stress as well as apoptoticike changes. It is concluded that FCM is an important tool in the research of sperm function, which helos to clarify the effect and mechanism of novel toical contraceotives.
出处
《生殖与避孕》
CAS
CSCD
2013年第1期48-54,共7页
Reproduction and Contraception
基金
上海市人口计生委项目(编号2012JG03)
国家自然科学基金项目(编号81100459)
上海市科学技术委员会项目(编号11140902900,11PJ1408400)
