摘要
为了解近年来辽宁地区猪瘟流行毒株的遗传变异情况,本试验利用RT-PCR方法对2006年～2011年辽宁地区发病猪群中猪瘟病毒感染情况进行了检测并获得了20株猪瘟病毒E2基因部分编码序列的扩增片段,测序后得到276bp的E2基因编码序列;并在此基础上利用DNAStar和MegAlign等软件对所测定的20株毒株与国内外参考毒株的相应序列进行了同源性分析及氨基酸序列比对。结果表明,20株猪瘟野毒株分别属于基因2.1、2.2亚型和1.1亚型,其中基因2.1亚型已经成为辽宁地区猪瘟病毒的优势流行毒株。属于基因2.1亚型的猪瘟野毒株与疫苗株之间的同源性在76.8%～80.5%之间,与经典强毒Shimen株的同源性在77.4%～81.1%之间。而病毒E2基因变异位点主要分布在所测序列的前30个氨基酸,与强毒Shimen株、疫苗株相比其变异率高达20%以上。说明近期流行毒株的变异呈现一定的多样性,并且多数毒株已向远离强毒Shimen株、疫苗株方向变异。
This study investigated genetic variation of classical swine fever virus （CSFV） strains isaolated from Liaoning province. The infection in piglets were diagnosed by reverse transcription polymerase chain reaction （RT- PCR） from year 2006 to 2011. Twenty strains of CSFV were selected to amplify E2 gene with PCR using specific primers, yielding an amplicon of 276 bp. DNA sequences of the fragment of E2 gene were compared with the corresponding sequences available in GenBank to determine their phylogenetic relationship. The phylogenetic analysis of the E2 genes showed that the twenty strains of CSFV isolates from Liaoning clustered in different branches, but was closer to the 2. 1, 2. 2 and 1.1 subtype respectively. 2. 1 subtype strain was more prevalent than other strains in Liaoning province. Their nucleotide homology were 77.4% to 80.5% and 77.4% to 81.1% as compared with vaccine strain and classic virulent Shimen strain. Mutations usually occurred in the first 30 amino acids of E2 protein with mutation rates over 20 %. The results indicated that the evolution of prevalent strains of CSFV is varied, and most strains are mutating far away from vaccine strain and classic virulent Shimen strain.
出处
《中国兽医杂志》
CAS
北大核心
2012年第12期6-10,共5页
Chinese Journal of Veterinary Medicine
