摘要
目的探讨丙戊酸钠对乳腺癌细胞Hs578T缝隙连接功能的影响及其可能机制。方法 MTT法检测丙戊酸钠的细胞毒性;细胞接种荧光示踪法测定Hs578T细胞之间荧光传递功能;Western blotting检测丙戊酸钠对Cx43总蛋白表达的影响;细胞免疫荧光法观察丙戊酸钠对Hs578T细胞膜Cx43蛋白表达的影响。结果 MTT检测结果显示丙戊酸钠在0-10 mmol/L浓度范围内对Hs578T细胞几乎无细胞毒性;细胞接种荧光示踪结果显示丙戊酸钠0-5 mmol/L显著增强Hs578T细胞荧光传递功能(P<0.01);Western blotting结果显示丙戊酸钠0-5 mmol/L可显著增强Cx43总蛋白表达(P<0.01);细胞免疫荧光结果显示丙戊酸钠0-5 mmol/L可显著增强Hs578T细胞膜Cx43蛋白的表达。结论丙戊酸钠能够显著增强乳腺癌细胞Hs578T的缝隙连接功能,这种增强作用可能与其增强Cx43总蛋白和细胞膜Cx43蛋白表达水平有关。
Obective To investigate the effect of valproate acid sodium （VPA） on gap junction intercellular communication in breast cancer Hs578T cells and explore the mechanism. Methods MTT assay was used to detect the cytotoxicity of VPA on Hs578T cells, and parachute assay was used to detect the effect of VPA on dye spread of the cells. Western blotting was employed to detect the expression changes of Cx43 total protein in VPA-treated Hs578T cells. The effect of VPA on the expression of Cx43 on the surface of Hs578T cells was examined with immunofluorescence assay. Results MTT assay showed no obvious cytotoxicity of VPA on Hs578T cells at the concentrations below 10 mmol/L. VPA below 5 mmol/L obviously increased the gap junction function in Hs578T cells （P〈0.01）, and significantly enhanced the expression of Cx43 total protein （P〈 0.01） and Cx43 expression on the surface of Hs578T cells （P〈0.01）. Conclusion VPA can obviously increase the gap junction function in Hs578T cells possibly by enhancing Cx43 total protein expression and Cx43 protein expression on the surface of Hs578T ceils.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第1期66-69,共4页
Journal of Southern Medical University
基金
国家自然科学基金(81001457)
安徽省教育厅省级优秀青年人才基金重点项目(2010SQRL121ZD)
安徽省教育厅高校省级科学研究项目(kj2010B117)~~
关键词
乳腺癌
丙戊酸钠
CX43
缝隙连接
breast cancer
valproate acid sodium
connexin43
gap junction
