摘要
环介导等温扩增(LAMP)技术是近年发展起来的一项新的快速核酸恒温扩增技术,具有高效、快速、特异、易检测、易操作等特点,自面世以来被科学家认为是能替代常规PCR的一项扩增技术,非常适用于现场检测和基层检测。本文就LAMP技术的原理、特点、进展及其在病原微生物检测中的应用进行了简要的概述。
Loop-mediated isothermal amplification(LAMP) technology is a novel nucleic acid isothermal amplification technology developed in recent years.Because of its high effiency,rapidity,specificity,and simplicity,scientists believe it will be a routine amplification technology replacing the conventional PCR method.This paper briefly reviews the principle of the technology,techinical characteristics,progress,and its application in pathogenic microorganism detection.
出处
《基础医学与临床》
CSCD
北大核心
2013年第1期19-23,共5页
Basic and Clinical Medicine
关键词
环介导等温扩增
检测
病原体
loop-mediated isothermal amplification
detection
pathogenic microorganism