摘要
为了解决蕙兰组织培养过程中分化速度慢的问题,以蕙兰名品‘大一品’自交种的根状茎为材料,系统研究基本培养基、植物生长调节剂、有机附加物、P/N比、碳源、蔗糖浓度6个因素对根状茎芽分化的影响。结果表明:MS培养基为蕙兰‘大一品’根状茎芽分化的最佳培养基;植物生长调节剂浓度配比对芽分化率影响显著,在含NAA0.3mg/L+6-BA2.0mg/L的培养基上芽分化率最高;有机附加物对根状茎芽诱导和分化有促进作用,添加100g/L香蕉泥能显著提高根状茎芽诱导率和分化率;减少培养基中N的含量(1/10N),增加P含量(5P),可以提高芽诱导率和分化率;麦芽糖为最适合芽诱导和分化的碳源;20g/L蔗糖对芽分化有显著的促进作用。
In order to study the rapid differentiation of rhizomes of Cymbidium faberi in tissue culture.The effects of basic medium,plant growth regulator,organic additives,P/N,carbon sources and sucrose concentration on the shoot differentiation of rhizomes were studied through the rhizomes from the self-cross seeds of Cymbidium faberi＇Dayipin＇.The results showed that the most appropriate cultivating medium was MS.The plant growth regulator had a significant effect on shoot differentiation.The highest shoot differentiation rate was appeared on 0.3 mg/L NAA＋ 2.0 mg/L 6-BA.The organic additives promoted the shoot induction rate and the shoot differentiation rate of the rhizomes,and supplement of 100 g/L mashed bananas in medium significantly improved the shoot induction rate and the differentiation rate.Reduced the content of N（1/10N） and increased the content of P（5P） in the medium,can improved the shoot induction rate and the differentiation rate.Maltose was the most suitable carbon source for improving the shoot induction rate and the differentiation rate of the rhizomes and 20 g/L sucrose promoted the shoot differentiation of the rhizomes significantly.
出处
《农学学报》
2012年第12期37-41,共5页
Journal of Agriculture
基金
国家公益专项"国兰新品种培育与产业化生产关键技术研究"(201004080)
关键词
根状茎
芽分化
影响因素
rhizome
shoot differentiation
influencing factors
