摘要
建立了反相高效液相色谱同时测定西红花苷Ⅰ和西红花酸的方法。采用C8色谱柱,流动相为甲醇水冰醋酸溶液,甲醇、水和冰醋酸比例为75∶24.5∶0.5,流速为0.6mL·min-1,检测波长为440nm,在该色谱条件下,西红花苷Ⅰ和西红花酸得到较好的分离,西红花苷Ⅰ和西红花酸浓度在0.625～40μg·mL-1范围内峰面积与进样量呈良好的线性关系,方法平均回收率为101.33%。该方法具有重复性好、准确、快速等优点,适用于西红花苷Ⅰ和西红花酸的同时测定。
In this study, we established a method for determination of crocin Ⅰ and crocetin simultaneously by re- verse phase High-Performance Liquid Chromatography. Elite C8 chromatographic column （4.6 mm ×250 mm, 5 μm） was used, the mobile phase was methanol water acetic acid solution, their proportion of methanol, water and acetic acid were 75： 24.5： 0.5, the detected wavelength was 440 nm, and the flow speed was 0.6 mL · min-1. The crocin Ⅰ and croeetin got better separation, both the contents of crocin Ⅰ and crocetin and their peak areas were in good linear relationships, respectively. The average recovery was 101.33%. This method showed good re- peatability, quantitative, accuracy and rapid, and could be used effectively for the simultaneous determination of crocin Ⅰ and crocetin.
出处
《东华理工大学学报（自然科学版）》
CAS
2012年第4期402-406,共5页
Journal of East China University of Technology(Natural Science)
基金
国家自然科学基金项目(30960046)
