摘要
为研究不同月份对绵羊卵母细胞采卵数及体外成熟的影响,采用切割法获得不同月份绵羊卵母细胞,统计分析可用卵母细胞数和体外培养成熟率。结果表明:随着月份的延续,卵母细胞获得数从5月的(3.53±0.27)(个/d)稳步增加到11月的(5.16±0.15)(个/d),差异极显著(P<0.01)。卵母细胞体外成熟率也显著提高,其中8月份成熟率极显著高于5月、6月、7月(P<0.01);9月、10月、11月卵母细胞成熟率又极显著的高于8月(P<0.01),同时季节和气温对绵羊卵母细胞采卵数和利用也有显著影响(P<0.01)。因此,不同月份采卵显著影响可用绵羊卵母细胞数和卵母细胞体外成熟水平。
Tbe subject of this research is to study the effects of month on the number of Cumulus-oocytes complexes （COCs） reclaim and oocytes maturation in sheep in vitro. The dissect way was used to collect oocytes from ovine ovary in different month, and the study was carried out by statistical analysis of the number of COCs reclaim and oocytes maturation rate. The results showed that the number of COCs reclaim increased from 3.53 ± 0.27/day of May to 5.16 ± 0.15/day of November （ significantly, P 〈 0.01 ） for the passing of time. The rate of oo- cytes maturation was also remarkablely improved. Oecytes maturation rate of August was significantly higher than that of May, June and July （ P 〈0.01 ）. While the percentages of maturation oecytes increased significantly in September, October and November compared to the August group （ P 〈 0.01 ）. At the same time, the season and temperature also had effects on the number of COCs reclaim and oocyles utilization siguificanfly （ P 〈 0.01 ）. Therefore, different months of collecting oocytes markedly affectted the number of COCs reclaim and oocytes maturation in sheep in vitro.
出处
《西南农业学报》
CSCD
北大核心
2012年第6期2347-2350,共4页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金资助项目(31060309)
中国博士后科学基金项目(2012M511579)
关键词
月份
卵丘卵母细胞
卵母细胞体外成熟
绵羊
Month
Cumulus-oocytes complexes, COCs
Oocytes maturatioa in vitro
Sheep
