摘要
以产乳糖酶米曲霉(ATCC20423)为出发菌株,通过紫外线和紫外复合氯化锂诱变处理选育高产乳糖酶菌株。利用紫外线辐照诱变得到突变株UV-15-20的乳糖酶活力为114.08U/mL,比出发菌株提高了49.22%;以紫外线复合氯化锂选育得到突变株UV-LiCl-38乳糖酶活力为121.42U/mL,比出发菌株提高了58.82%。传代实验表明两个突变株均具有稳定的遗传性。
In order to breed high-yield β-Galactosidase producing strain,Aspergillus Oryzae（ATCC20423）was used as the original strain and mutagenized with Ultraviolet（UV）and UV＋lithium chloride（LiCl）,respectively.After mutagenized by UV,the β-Galactosidase activity of UV-15-20 reached 114.08U/mL,which revealed a 49.22% increase compared with the original strain.A mutant UV-LiCl-38 with high β-Galactosidase activity（121.42U/mL）was obtained by use of UV and LiCl,the β-galactosidase activity of this mutant was 58.82% higher than that by the wild-type.UV-15-20 and UV-LiCl-38 had good hereditary stability after several generations.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第1期155-159,共5页
Science and Technology of Food Industry
基金
甘肃省科技重大专项(1002FKDA046)
甘肃省农业生物技术专项(GNSW-2010-17)
兰州市政研产合作支撑计划项目(2011-1-105)
关键词
米曲霉
乳糖酶
诱变
Aspergillus Oryzae
β-Galactosidase
mutation
