摘要
目的:观察健膝丸对膝骨关节炎模型大鼠的软骨光镜下组织形态学的影响。方法:将40只大鼠随机区组法分为正常组、模型组、实验组(健膝丸组)、对照组(抗骨增生胶囊组)。按照改良Hulth方法建立动物模型。用药8周后处死动物取材,分别经HE、Masson、Safranin-O染色,光镜观察并行Mankin's评分。结果:光镜下观察实验组、对照组及模型组均有软骨破坏,模型组更为明显。Mankin's评分,与正常组比较,其余3组积分显著增加(P<0.05),实验组、对照组与模型组相比均有显著性差异(P<0.05),实验与对照组相比无显著性差异(P>0.05)。结论:健膝丸可减少软骨Ⅱ型胶原和蛋白多糖丢失,减轻关节软骨破坏,减缓软骨下骨硬化,延缓骨关节炎的发展进程,但同时表明其不能停止或逆转关节软骨的退变。
Objective： To observe the cartilage Histomorphology effect of Jianxi pill on the osteoarthritis（OA）-model rats. Methods：40 4-month-old Sprague Dawley rats were divided randomly and equally into 4 groups： the normal group, the model group, the experimental group （the Jianxi pill group） and the control group （the Kangguzengsheng capsule group）. The normal group was treated with sham operation and the other groups were duplicated by modified Hulth＇s technique. All rats were killed after 8 weeks. Specimens were procured from articular cartilage of femoral condyle and subchondral bone, which were examined under light microscope after using HE staining and Masson trichrome staining and Safranin-o-fast green-iron staining, and the histological changes of them were graded by Mankin＇s scale. Results： Under light microscope after using staining, the articular cartilage degenerative changes of the model group were obvious, and improvement were found in the experimental group and the control group. The Mankin＇s grade of the model group was significantly higher than those of the experimental group and the control group （P 〈 0.05）, and no significant difference was found between the experimental group and the control group （P 〉 0.05）. Conclusion： Jianxi pill could reduce the lose of proteogly and collagen 1[ and the damage of articular cartilage, slow the hardening process of the subchondral bone and delay the development of the ostarthritis, showing that it could not stop and deteriorate the degeneration of articular cartilage.
出处
《风湿病与关节炎》
2013年第1期13-17,共5页
Rheumatism and Arthritis
关键词
骨关节炎
膝
健膝丸
中医药疗法
关节软骨
组织形态学
实验研究
动物
大鼠
osteoarthritis,knee
jianxi pill
TCM treatment
articular cartilage
histomorphology
experimental study
animal
rat
