摘要
目的研究高效抗反转录病毒治疗(HAART)1年,对HIV/AIDS患者免疫重建效果的影响。方法确认人免疫缺陷病毒(HIV)感染者35例,检测CD4+T淋巴细胞<350/μL,开始HAART治疗,并于治疗0、6、12个月时分别检测其病毒载量及CD4+T淋巴细胞、CD8+T淋巴细胞、CD4+童贞细胞、CD4+记忆细胞、CD8+激活细胞计数。结果治疗前,HIV RNA处于高复制水平,平均(4.62±1.09)×106copies/mL,HAART治疗6个月及12个月时均低于检测下限。患者的CD4+T淋巴细胞、CD4+童贞细胞和CD4+记忆细胞均明显增加,12个月时,各组细胞计数[分别为(312±109.22)/μL、(150±57.34)/μL、(212±48.06)/μL]与基线[分别为(183±83.73)/μL、(73±20.40)/μL、(119±30.42)/μL]比较,差异有统计学意义(P<0.01),但仍低于健康对照组[分别为(768±146.41)/μL、(424±87.06)/μL、(442±61.40)/μL](P<0.05);CD8+T淋巴细胞计数(427±99.79)/μL较基线(597±111.43)/μL明显下降(P<0.05),但仍高于健康对照组(208±37.39)/μL(P<0.05)。结论 HAART能快速抑制HIV复制,治疗1年能部分恢复HIV/AIDS患者免疫功能。
Objective To investigate the impact of one-year highly active antiretroviral therapy （HAART） on an- tiviral effect and immune reconstruction in HIV/AIDS patients. Methods Thirty-five HIV/AIDS patients were initially treated with HAART when CD4 ＋ T cells were 〈350/μL, blood samples were taken at the HAART time point 0,6,12 months, the virus load was tested by RT-PCR, CD4 ＋ T cells, CD8 ＋ T cells, CD4＋ naive cells, CD4 ＋ memory cells and CD8 ＋ activated cells were detected by flow-cytometry. Results The average virus load of 35 patients was （4. 62 ± 1.09） × 10^6 copies/mL before HAART, and was lower than the lower limit of detection value after HAART for 6 months and 12 months. The levels of CD4 ＋ T cells, CD4 ＋ naive cells and CD4 ＋ memory cells were （312 ± 109. 22） /μL, （150 ± 57. 34）/μL and （212 ± 48. 06）/μL respectively after HAART for 12 months, while they were （183 ± 83. 73）/μL, （73 ± 20. 40）/μL and （119 ± 30. 42）/μL respectively at the baseline （P〈0. 01）, but they were still lower than healthy control group（[768± 146. 41]/μL, [-424± 87. 06]/μL and [442 ± 61.40]/μL, respectively） （P〈0. 05） ;the level of CD8 ＋ T cells were（427 ± 99. 79）/μL after HAART, which decreased significantly compared with （597 ± 111.43）/μL of the baseline （P〈0. 05）, but still higher than （208 ± 37. 39）/μL of healthy control group （P〈0. 05）. Conclusion HAART can both inhibit virus replication rapidly and reconstruct HIV/AIDS patients＇ immunity partially.
出处
《中国感染控制杂志》
CAS
2012年第6期409-412,共4页
Chinese Journal of Infection Control
基金
中南大学研究生教育创新工程项目(2011ssxt177)
