摘要
目的:探讨miRNA-375的外源性表达对人胰腺癌细胞Panc-1增殖和凋亡的影响。方法:将miRNA-375表达质粒或对照质粒转染Panc-1细胞,qRT-PCR方法检测miRNA-375在转染细胞中的表达水平,细胞计数法、流式细胞术分别检测外源性miRNA-375表达后Panc-1细胞增殖、凋亡和周期的变化情况。结果:miRNA-375表达质粒组与对照组比较,其miRNA-375表达诱导性升高,细胞增殖受到抑制,细胞凋亡率增加,细胞周期阻滞于G0～G1期。结论:在胰腺癌细胞Panc-1中增加miRNA-375的表达,可抑制细胞增殖,促进细胞凋亡和细胞周期阻滞,miRNA-375在胰腺癌细胞Panc-1中可能发挥潜在的抑癌基因作用。
Objective： This study aimed to investigate the effect of the ectogenesis expression ofmiRNA-375 on cell proliferation and apoptosis in the human pancreatic cancer cell line Panc-1. Methods： Aiier transfection of the miRNA-375 vector, the expression levels of miRNA-375 in the Panc-1 cells were determined by quantitative real-time polymerase chain reaction. Cell counting was performed to evaluate the effects of miRNA-375 expression on cell proliferation. Flow cytometry was used to analyze apoptosis and the cell cycle. Results： The expression levels of miRNA-375 were highly induced after miRNA-375 transfection, miRNA-375 restoration also inhibited cell proliferation, promoted cell apoptosis, and induced cell cycle arrest at G0-G1. Conclusion： The abnormal expression of miRNA-375 in the pancreatic cancer cell line Panc- 1 inhibits the canceration of cells by interfering with cellular biological behaviors, such as proliferation and apoptosis, miRNA-375 may act as a potential tumor suppressor in the pancreatic cancer cell Panc-1.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2013年第1期21-24,共4页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金青年项目(编号:81201905)资助
