摘要
目的探讨鞘氨醇激酶1(SphK1)在胃癌发生、发展中的作用及其与血管内皮生长因子(VEGF)的相关性。方法收集2011年5月至2011年8月经手术切除的31例胃癌及其癌旁组织标本,用实时荧光定量PCR法检测组织标本中SphK1 mRNA的表达量;免疫组化法检测SphK1和VEGF的蛋白表达水平。结果 SphK1 mRNA在胃癌织中的表达量为2.77±0.50,高于相应癌旁组织的1.02±0.23(P<0.05)。胃癌组织SphK1蛋白的高表达率为71.0%(22/31),高于相应癌旁组织的38.7%(12/31),差异有统计学意义(P<0.05)。SphK1蛋白与mRNA的表达分别与浸润深度、TNM分期、淋巴结转移及血管侵犯相关,而与年龄、性别及分化程度无关。胃癌组织VEGF蛋白的高表达率为64.5%(20/31),与SphK1蛋白表达呈正相关(r=0.565,P<0.05)。结论 SphK1在胃癌组织中高表达,并可能与VEGF共同促进胃癌的发生和发展。
Objective To investigate the role of Sphingosine kinase-1 （ SphK1 ） in the occurrence and progression of gastric cancer, and its correlation with vascular endothelial growth factor（VEGF）. Methods Quantitative real-time reverse transcription-PCR （qRT-PCR） assay was used to determine the expression of SphKl mRNA in 31 cases human gastric cancer tissues and paracarcinoma tissues. Immunohistochemical method（SP） was used to detect the expression of SphK1 and VEGF protein. Results The expression of SphK1 mRNA in gastric cancer and corresponding paracarcinoma tissues was 2. 77 -＋ O. 50 and 1.02 ＋-0. 23 （P 〈 0. 05 ）. The high ex- pression rate of SphK1 protein in the gastric cancer tissues was 71.0% （22/31） , higher than 38.7% （ 12/31 ） in the corresponding pa- racarcinoma tissues（P 〈 O. 05）. The expression of SphK1 in gastric cancer tissues was correlated with the depth of infiltration, TNM clinical stage, lymph node metastasis and vessel invasion, but not with age, gender and differentiation. The high expression rate of ~EGF protein in the gastric cancer tissues was 64. 5% （20/31）. The expression of SphK1 protein were positively related with VEGF protein in gastric cancer（r =0. 565, P 〈 0. 05）. Conclusion SphKI highly expresses in gastric cancer, and promote the oecurrenee and progression of gastric cancer in correlation with VEGF.
出处
《临床肿瘤学杂志》
CAS
2012年第12期1076-1080,共5页
Chinese Clinical Oncology
关键词
胃癌
鞘氨醇激酶-1
血管内皮生长因子
Gastric cancer
Sphingosine kinase-1
Vascular endothelial growth factor
