摘要
目的探讨模拟不同海拔高原环境下完成氦氧潜水对潜水员应激反应的影响。方法4名潜水员于高、低压舱内，模拟海拔3000m环境下潜水30m停留60rain，连续2d；4000m环境下潜水30m停留60min，连续2d；5200m境下潜水30m停留60min，1d；5200m环境下潜水50m停留60min，1d；整个实验于密闭高、低压舱内连续完成，共9d。于潜水员进舱前、第1次潜水前、第2次、第4次潜水后、第5次潜水前、第6次潜水后及出舱后分别留取静脉血，采用酶联免疫（ELISA）法测定去甲。肾上腺素（NE）、5-羟色胺（5-HT）、多巴胺（DA）含量。结果外周血5-HT含量先缓慢升高，在经历海拔3000m潜水30m2d、4000m潜水30m2d后达到最高值[（3．24±0．66）μg/L]，之后下降，至5200m第2次潜水（30m、50m各1次）后降至最低值[（2．50±1．18）μg/L]，其变化比较差异无统计学意义（P〉0．05）；DA变化基本与5-HT一致；外周血NE含量首先下降，在经历3000111潜水30m^2次后开始升高，再次经历4000m^2次潜水后达到最高值，之后持续下降，直至出舱后达到最低值，其变化比较差异无统计学意义（P〉0．05）。结论模拟高海拔潜水使机体产生应激反应，程度处于应激反应的警觉期及抵抗期，尚在生理调节范围之内。
Objective To investigate effects of simulated heliox diving on the stress of the divers at different high altitudes. Methods In the dual-purpose hyperbaric and hypobaric chamber complex, 4 divers conducted a series of simulated dives at various altitudes and under different simulated environmental conditions ： the 30 m dive at an altitude of 3000 m for a duration of 2 days, the 30 m dive at an altitude of 4000 m for a duration of another 2 days, and the 30 m dive at an altitude of 5200 m for a duration of just 1 day, and the 50 m dive at an altitude of 5200 m also for a duration of just 1 day. Blood samples were taken respectively at the following time points： i.e. before entrance into the chamber; before the first dive; after termination of the first and fourth dives ; before the fifth dive and after the sixth dive. Levels of norepinephrine （NE）, 5-hydroxytryptamine （5-HT） and dopaminum （DA） were detected with ELISA. Results At first, levels of 5-HT increased gradually, reached peak following 2 dives respectively at the altitudes of 3000 m and 4000 m, and then decreased to the lowest level after 2 dives （ one 30 m dive and one 50 m dive） at an altitude of 5200 m. However, no statistical significance could be detected in the changes mentioned above. Changes in the levels of DA were almost the same as those of 5-HT. Levels of NE first decreased, then began to increaseafter two 30 m dives at the altitude of 3000 m, reached the peak following two dives at the altitude of 4000 m, and then decreased gradually and reached the lowest level when the divers came up to chamber surface. Statistical significance in the changes could neither be observed in the levels of NE. Conclusions Simulated high altitude heliox diving could induce stress response of the body, the extent of which depended on the phases of ＂vigilance＂ and ＂resistance＂, and it was not beyond the physiological regulation of the body.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
北大核心
2012年第6期325-326,330,共3页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
基金
总装备部装备技术基础项目（YXSJD20101BZ01）
关键词
模拟高海拔
氦氧潜水
应激
Simulated altitude
Heliox diving
Stress
