摘要
目的:采用衍生化反应,建立荧光分光光度法测定中药中二氧化硫的残留量。方法:对影响荧光强度的因素进行研究,确定最佳衍生条件。在激发波长321 nm,发射波长384 nm处测定荧光强度。结果:荧光强度与Na2SO3对照品加入量在0.999 7～17.99 nmol线性关系良好,r=0.999 9,平均回收率102.3%,RSD 4.6%。结论:该法具有简便易行,快速准确,灵敏度高等特点,可为中药材二氧化硫残留量的测定提供参考。
Objective： To establish a derivative fluorometry method for the determination of sulfur dioxide residues in tradition- al Chinese medicine. Method： The optimal derivation condition was established. The fluorescence intensity was detected at excitation wavelength of 321 nm, and emission wavelength of 384 nm. Result： A linear relationship was obtained between the fluorescence inten- sity and the addition of reference substance in the range of 0. 999 7-17.99 nmol with a correlation eoeffient of 0. 999 9, and the average recovery was 102.3% with RSD 4. 6%. Conclusion： This method is simple and sensitive with quick and correct result. It can provide a reference for the determination of sulfur dioxide residues in traditional Chinese medicine.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第2期212-216,共5页
China Journal of Chinese Materia Medica
基金
四川省质量监督局重要技术标准研究项目(ZY-BZ2011-50)
关键词
荧光分光光度法
衍生
二氧化硫残留量
中药
fluorescence spectrophotometry
derivative
sulfur dioxide residues
traditional Chinese medicine
