摘要
采用紫外线和氯化锂复合诱变技术,对烟酰胺产生菌Rhodococcus.equi的原生质体进行了诱变育种。原生质体制备和再生的最佳条件为:甘氨酸浓度2.6%,菌龄36 h,酶浓度2.0 mg/mL,酶解温度32℃,酶解时间40 min,原生质体的形成率达到94.6%。复合诱变的最佳条件:紫外线照射80 s,氯化锂浓度0.6%。最终获得一稳定高产3-氰基吡啶水合酶的菌株Rhodococcus.equi LY-PM09,其产酶酶活性为1 247 U/mL,比出发菌株提高了91.3%。
Using UV and LiCl mutagenesis technology, nicotinamide bacteria Rhodococcus. equi protoplast conduct mutation breeding. The best conditions of protoplast formation and regeneration are namely gly- cine concentration 2.6% ,fungus age 36 h, enzyme concentration 2.0 mg/mL, enzyme solution tempera- ture 32 ℃, enzymolysis time 40 min, protoplast formation rate reached 94.6%. The best conditions of compound mutation are as follows ： ultraviolet irradiation 80 s, lithium chloride concentration 0.6%. Final- ly ,the study get a stable-high-yield strains of 3-cyanopyridine hydratase, Rhodococcus equi LY-PM09, the enzyme production enzyme activity is 1 247 U/mL,which increased 91.3% than starting strain.
出处
《应用化工》
CAS
CSCD
2013年第1期72-76,共5页
Applied Chemical Industry
